Key factors for causing poplar Ice Nucleation Active bacterial canker and its control techniques

Abstract

The isolation, culture and the active determination of poplar ice nucleation active (INA) bacteria and the inoculation tests in laboratory and field were conducted, and the varieties, distribution and number of poplar INA bacteria and its pathogenicity and freezing injury property were determined. The study results showed that the INA bacteria widely spread on poplar in Northeast China and caused the frozen injury for poplar under the frost condition in Spring or Autumn, which was the key factor to induce INA bacterial canker. Through evaluation and investigation of different poplar varieties and inoculation tests, fine disease-resistant varieties and strains of poplar suitable for Northeast China were selected. Further tests for strong seedling showed that burying cuttings in sand and covering with plastic film could effectively avoid the frostbite, frozen and drought damage, reduce INA bacteria infection, and promote poplar growth. INA bacterial canker was detected early by highly specialized antiserums of INA bacteria and the agglutinated test of ring-shaped boundary surface. The inducers such as streptomycin, phenylmercuric acetae, salicylic acid and heat-killed bacteria to immerse cuttings, have obvious induced disease-resistant effect. Before poplar sprouted in early spring, through spraying the solution of frostbite agent, the control effect also was obvious.

Keywords

S763.1 / S792.11 / A

References

[1] Bacterial classification group of Microbe research institute of Chinese scientific graduate school Common determination method for general bacteria [M], 1978 Beijing: Scientific Press 135-193.

[2] Miaoying Cai, Skerman V.B.D. Determination instruction of bacteria [M], 1992 Beijing: Scientlfu Press 129-199.

[3] Zhongda Fang Research methods of plant diseases [M], 1997 Beijing: Agriculture Press 269-275.

[4] Xuefeng Liu, Airong Dong, Fengxian Su et al. The comment and investigation on regional test of poplar fine varieties [J]. Journal of Northeast Forestry University, 1999, 27(1): 71-74.

[5] Xunsheng Liang Serological technique of plant diseases [M], 1985 Beijing: Agriculture Press 131-133.

[6] Li Chunjiang, Xiang Chunti, Mao Dejiang,et al. 1996. Study on hosts’ induced resistance on polar INA bacterial canker. Forest research, (Special): 184.

[7] Translation group of Beijing bacterial determination manual Buchanan R.E., Gibbons N.E. Beijing bacterial determination manual [M], 1984 Beijing: Scientific Press 274-486.

[8] Ping Wang Contrast test on growing condition and resistance of several main poplars [J]. Jilin Forestry Science and technology, 1996, 30: 15-17.

[9] Cunti Xiang Research methods of forestry disease [M], 1991 Harbin: North-east forestry university Press 269-275.

[10] Cunti Xiang, Liping Shao Integrated protect and control technique of poplar disease [M], 1992 Harbin: The press of Northeast Forestry University 119-172.

[11] Xiang Cunti, Song Fugiang, Liu Jinghua,et al. 2001a. The dominant factors caused by INA bacterial canker [J]. Journal of Northeast Forestry University, 109–113

[12] Cunti Xiang, Gongbin Li, Airong Dong et al. Screening for the resistant improved varieties of poplar ice nucleation bacterial canker [J]. Journal of Northeast Forestry University, 2001, 29(3): 123-127.

[13] Cunti Xiang, Gongbin Li, Airong Dong et al. Control techniques of poplar INA bacterial canker [J]. Journal of Northeast Forestry University, 2001, 29(3): 134-138.

[14] junhai Yao Serological technique use in forestry disease research [J]. Forest pest and disease, 1990, 4: 36-36.