Shoot multiplication of Syringa reticulata var. mandshurica from in vitro cultured seedlings

Abstract

We developed a shoot multiplication protocol for Syringa reticulata Blume var. mandshurica Hara from in vitro cultured seedlings that derived from in vitro germinated seeds. The shoots could be induced on Murashige and Skoog (MS) medium with proper plant growth regulator combinations of 6-benzylaminopurine (BA) and indole-3-butyric acid (IBA). The better medium for shoot multiplication and growth was MS + 5 mg L⁻¹ BA + 0.5 mg L⁻¹ IBA + 20 g L⁻¹ sucrose + 7 g L⁻¹ agar, and the corresponding shoot induction rate was 75 %. The plantlets grew well after rooting on 1/2MS medium (macro-elements of MS medium are at half-strength) supplemented with 1 mg L⁻¹ IBA, and the survival percentage was >80 % at 16 weeks after transplanting.

Keywords

Manchurian lilac / Micropropagation / Plant growth regulator / Shoot multiplication / Sucrose concentration

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