Neofusicoccum parvum causing canker of seedlings of Juglans regia in China

Abstract

Cankered, dying seedlings of Juglans regia were observed in Shaanxi province in the northwest region of China. Neofusicoccum parvum was isolated from these cankered tissues, with the identification based on morphology and an ITS-nrDNA sequence. In order to demonstrate how cultures of N. parvum could cause the expected symptoms, artificial infection, using these isolates and re-isolation of the pathogen, was used. This is the first report on this taxon as a walnut canker pathogen in China.

Keywords

Forest tree disease / ITS-nr-DNA / Koch’s postulates / Neofusicoccum parvum / Walnut

References

[1] Denman S, Crous PW, Groenewald JZ, Slippers B, Wingfield BD, Wingfield MJ. Circumscription of Botryosphaeria species associated with Proteaceae based on morphology and DNA sequence data. Mycologia, 2003, 95(2): 294-307.

[2] Hagsag WM, Rayya A, Kasim NE. First report of a canker disease of walnut caused by Botryodiplodia theobromae in Egypt. Plant Dis, 2007, 91 2 226.

[3] Inderbitzin P, Bostock RM, Trouillas FP, Michailides TJ. A six locus phylogeny reveals high species diversity in Botryosphaeriaceae from California almond. Mycologia, 2010, 102: 1350-1368.

[4] Maas JL, Uecker FA. Botryosphaeria dothidea cane canker of thornless blackberry. Plant Dis, 1984, 68: 720-726.

[5] Martos S, Torres E, Bakali MA, Raposo R, Gramaje D, Armengol J, Luque J. Co-operational PCR coupled with dot blot hybridization for the detection of Phaeomoniella chlamydospora on infected grapevine wood. J Phytopathol, 2011, 159(4): 247-254.

[6] Michailidis TJ. Pathogenicity, distribution, sources of inoculum, and infection courts of Botryosphaeria dothidea on pistachio. Phytopathology, 1991, 81: 566-573.

[7] Michalidis TJ, Chen SF, Coates B, Morgan D, Puckett R, Hasey J, Anderson K, Buchner R, DeBuse C, Fichtner E, Bentley W. Managing anthracnose blight and Botryosphaeria and Phomopsis cankers of Walnut. 2012, Research Reports: Walnut, 367 381

[8] Moral J, Munoz-Diez C, Gonzalez N, Trapero A, Michailides TJ. Characterization and pathogenicity of Botryosphaeriaceae species collected from olive and other hosts in Spain and California. Phytopathology, 2010, 100(12): 1340-1351.

[9] Nei M, Kumar S. Molecular evolution and phylogenetics. 2000, New York: Oxford University Press

[10] Pennycook SR, Samuels GJ. Botryosphaeria and Fusicoccum species associated with ripe fruit rot of Actinidia deliciosa (kiwifruit) in New Zealand. Mycotaxon, 1985, 24: 445-458.

[11] Perez CA, Wingfield MJ, Slippers B, Altier NA, Blanchette RA. Endophytic and canker-associated Botryosphaeriaceae occurring on non-native Eucalyptus and native Myrtaceae trees in Uruguay. Fungal Divers, 2010, 41(1): 53-69.

[12] Phillips AJL, Fonseca F, Povoa V, Castilho R, Nolasco G. A reassessment of the anamorphic fungus Fusicoccum luteum and description of its teleomorph Botryosphaeria lutea sp. nov. Sydowia, 2002, 54: 59-77.

[13] Rumbos IC. Twig and branch dieback of walnut trees induced by Botyosphareria ribis. Plant Pathol, 1987, 36: 602-605.

[14] Sakalidis ML, Hardy GEJ, Burgess TI. Endophytes as potential pathogens of the baobab species Adansonia gregorii: a focus on the Botryosphaeriaceae. Fungal Ecol, 2011, 4: 1-14.

[15] Slippers B, Crous PW, Denman S, Coutinho TA, Wingfield BD, Wingfield MJ. Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea. Mycologia, 2004, 96: 83-101.

[16] Slippers B, Johnson GI, Crous PW, Coutinho TA, Wingfield BD, Wingfield MJ. Phylogenetic and morphological re-evaluation of the Botryosphaeria species causing diseases of Mangifera indica. Mycologia, 2005, 97(1): 99-110.

[17] Smith CO. Inoculations showing the wide host range of Botryosphaeria ribis. J Agric Res, 1934, 49: 467-476.

[18] Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol, 2007, 24(8): 1596-1599.

[19] Tang W, Ding Z, Zhou ZQ, Wang YZ, Guo LY. Phylogenetic and pathogenic analyses show that the causal agent of apple ring rot in China is Botryosphaeria dothidea. Plant Dis, 2012, 96(4): 486-496.

[20] Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 1997, 25: 4876-4882.

[21] Urbez-Torres JR, Peduto F, Striegler RK, Urrea-Romero KE, Rupe JC, Cartwright RD, Gubler WD. Characterization of fungal pathogens associated with grapevine trunk diseases in Arkansas and Missouri. Fungal Divers, 2012, 52(1): 169-189.

[22] von Arx JA, Muller E. Re-evaluation of bitunicate ascomycetes with keys to families and genera. Study Mycol, 1975, 9: 1-159.

[23] Whitch W, Clayton CN. Blueberry stem blight caused by Botryosphaeria dothidea (B. ribis). Phytopathology, 1963, 53: 705-712.

[24] White TJ, Bruns T, Lee S, Taylor J. Innes MA, Gelfand DH, Sninsky JJ, White TJ. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: a guide to methods and applications. 1990, San Diego: Academic Press

[25] Yu L, Chen XL, Gao LL, Chen HR, Huang Q. First report of Botryosphaeria dothidea causing canker and shoot blight of Eucalyptus in China. Plant Dis, 2009, 93 7 764

[26] Yu ZD, Zhao GC, Dan JY, Ren ZZ. Phylogeny of Botryosphaeria species based on ITS-nrDNA sequences. Mycosystema, 2010, 29: 285-293.

[27] Yu ZD, Wang JL, Tang GH, Zhai MZ. Exploring of endophytic Bacillus subtilis as an agent of biocontrol for walnut canker. Biotechnol Indian J, 2015, 11(11): 419-425.