Expression analysis of ThGLP, a new germin-like protein gene, in Tamarix hispida

Abstract

Germin and Germin-like protein (GLP) have various proposed roles in plant developmental stages and stress- related processes. A novel GLP cDNA clone was isolated from a cDNA library of Tamarix hispida. ThGLP, coded 225aa, possesses conserved motif of plant germin and Germin-like protein. ThGLP belongs to true germin subfamily through phylogenetic analyses. Gene expression profiles in roots and leaves were evaluated using real-time quantitative RT-PCR. The results show that the gene was highly induced by drought, salt, low temperature, CdCl₂ and abscisic acid treatments. Our results demonstrate that the ThGLP gene is expressed in leaves and roots, is involved in different abiotic stress responses and controlled by an ABA-dependent signaling pathway.

Keywords

germin-like protein (GLP) / Tamarix hispida / abiotic stress / gene expression

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