RAPD analysis of genetic diversity of nine strains of Auricularia auricular cultivated in Heilongjiang Province
Ling Li , Jie Li , Li Zou , Su-ying Bai , Li-ming Niu , Yu-kun Ma
Journal of Forestry Research ›› 2007, Vol. 18 ›› Issue (2) : 136 -138.
RAPD analysis of genetic diversity of nine strains of Auricularia auricular cultivated in Heilongjiang Province
Polymorphism of nine strains (CF05, CF09, 29, 916, AU9, Chang10, Chang7, 8808 and AU. Japanese) of A. auricular cultivated in Heilongjiang Province were analyzed by RAPD (Random Amplication polymorphic DNA). Thirteen primers were selected from forty PCR primers with 10bp long random primer. The results showed that nine strains of A. auricular have a high level of genetic diversity and the percentage of DNA polymorphic was 96.05. The genotypes of 9 strains of Auricularia auricular were identified by the fingerprints from primer 27 and primer 46 by RAPD analysis. The results are helpful for quickly identifying strains of A. auricular in its early breeding time, and also provides a powerful theoretic basis to differentiate strains (Auricularia auricular) whose morphology is very similar in breeding programs of edible fungus.
Auricularia auricular / RAPD analysis / DNA fingerprint / Genetic diversity
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