Dynamic change of protein polypeptide of Ginkgo biloba seed during germination

Abstract

The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis (2-DE). The results showed that 80 kinds of protein spots in endosperms of Gingkgo biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26–52kD, and their isoelectric points were in the range of 5.8–7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of proteins were synthesized; 7 kinds of proteins with different molecular weights and isoelectric points of 35kD/pI6.8, 31kD/pI6.8, 29kD/pI6.8, 33kD/pI6.6, 33kD/pI 6.4, 34kD/ pI7.7 and 31kD/pI7.7 were identified primarily as vegetative storage proteins (VSPs).

Keywords

Ginkgo biloba seed / Germination / Protein / Electrophoresis

References

[1] Cao B., Cai C., Mu H. Post-maturity physiology of the Ginkgo seed nuts Economic Forest Research, 2002, 3: 20-23.

[2] Cao B., Li Y., Zhou S. The primary study of physiology during the germination of Ginkgo seed Journal of Shandong Forestry Science & Technology, 1995, 1: 11-14.

[3] Fu J. The seed physiology, 1985 Beijing: The Science Press 23-60.

[4] Li S., Liu S., Zhao D. Biochemistry, 2005 Harbin: Harbin Technology University Publishing House 115-139.

[5] Lin L., Fu J. Relationship between the accumulation of storage proteins and formation of vigor of peanut seed Journal of Tropical and Subtropical Botany, 1995, 4(1): 57-60.

[6] Lin L., Fu J. Relationship between the activity of the peptidase and vigor of peanut seed Chinese Oil Crops, 1995, 17(3): 22-24.

[7] Liu J., Huang S., Fu J., Tang X. Advances on relation between seed vigor and proteins Chinese Bulletin of Botany, 2001, 18(1): 46-51.

[8] Peng F., Guo J. Progresses of research on vegetative storage proteins in woody plants Chinese Bulletin of Botany, 2001, 18(4): 445-450.

[9] Santos C., Ferreira R. Preliminary characterization of a glycosidase possibly involved in the mobilization of storage proteins from lupinus albus seed European Association for Grain Legume Research, 1998, 11: 296-297.

[10] Shatter R.A. Identification of biotinylated proteins in soybean (Glycine max merrill) seeds and their characterization during germination and seeding growth Seed Science Research, 1997, 7(4): 373-376.

[11] Tian W., Yan X., Hu Z. Cytological identification of vegetative storage protein in the new shoots of Poplar Acta Bot. Boreal-occident Sin., 2003, 23(7): 1143-1147.

[12] Wang X. Progress of store protein of wheat seed Seed, 1992, 57(1): 37-39.

[13] Xu L., Zhao M. The decomposition of storage protein of legume seed Seed, 1992, 100(1): 37-39.

[14] Yang B., Chen D. The conformation and the accelerating germination technique of Gingko biloba seed Journal of Seed Science and Technology, 2000, 4: 229

[15] Yu S., Wang H., Zhu N. Introduction to immunology, 2004 Beijing: The science publishing house 13-60.