Dynamic change of protein polypeptide of Ginkgo biloba seed during germination

Hong-yan Guo; Sheng-ping Li; Fang-ren Peng

doi:10.1007/s11676-007-0007-y

Journal of Forestry Research ›› 2007, Vol. 18 ›› Issue (1) : 35 -38. DOI: 10.1007/s11676-007-0007-y

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Dynamic change of protein polypeptide of Ginkgo biloba seed during germination

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Abstract

The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis (2-DE). The results showed that 80 kinds of protein spots in endosperms of Gingkgo biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26–52kD, and their isoelectric points were in the range of 5.8–7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of proteins were synthesized; 7 kinds of proteins with different molecular weights and isoelectric points of 35kD/pI6.8, 31kD/pI6.8, 29kD/pI6.8, 33kD/pI6.6, 33kD/pI 6.4, 34kD/ pI7.7 and 31kD/pI7.7 were identified primarily as vegetative storage proteins (VSPs).

Keywords

Ginkgo biloba seed / Germination / Protein / Electrophoresis

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Hong-yan Guo, Sheng-ping Li, Fang-ren Peng. Dynamic change of protein polypeptide of Ginkgo biloba seed during germination. Journal of Forestry Research, 2007, 18(1): 35-38 DOI:10.1007/s11676-007-0007-y