To obtain strains capable of efficient cembratriene-4,6-diol(CBT-diol)degradation for enhancing the tobacco aroma quality, a target strain designated as Z1-1 was isolated from tobacco leaves utilizing CBT-diol as the primary carbon source. Based on morphological characteristics, physiological biochemistry, and 16S rRNA gene sequence analysis, the strain was preliminarily identified as Novosphingobium sp. Through single-factor and response surface methodology experiments, the optimal degradation conditions were determined to be 30 ℃, 150 r/min shaking speed, 4% inoculum size, and pH 7. 0. Under these optimized conditions, a CBT-diol degradation efficiency of 83. 47% was achieved within 36 h. The application of this strain in tobacco concentrate significantly increased the relative contents of several key aroma components. The relative contents of solanone, 9-hydroxy-4, 7-megastigmatrienone, 4-hydroxy-β-damascone, and phytol increased to 7. 22, 3. 65, 1. 79, and 3. 80 times their original levels, respectively. These findings demonstrate that Novosphingobium sp. Z1-1 is an efficient CBT-dioldegrading and aroma-producing bacterium.
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Funding
Open Project of the Key Laboratory of Cigarette Smoke, China National Tobacco Corporation(K2023-1-044P)
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