A panoramic view of the virosphere in three wastewater treatment plants by integrating viral-like particleconcentrated and traditional non-concentrated metagenomic approaches
Jiayu Zhang , Aixi Tang , Tao Jin , Deshou Sun , Fangliang Guo , Huaxin Lei , Lin Lin , Wensheng Shu , Pingfeng Yu , Xiaoyan Li , Bing Li
iMeta ›› 2024, Vol. 3 ›› Issue (3) : 188
A panoramic view of the virosphere in three wastewater treatment plants by integrating viral-like particleconcentrated and traditional non-concentrated metagenomic approaches
•This study comprehensively compared viral-like particle-concentrated (VPC) and non-concentrated (NC) metagenomic approaches in virome investigation, highlighting their respective efficacies in detecting different viral communities and functional elements in wastewater treatment plants (WWTPs). •Eukaryotic viruses belonging to Cressdnaviricota were the most prevalent in VPC metagenomes, while bacterial viruses belonging to Uroviricota were the most abundant in NC metagenomes. •Diverse phage-born auxiliary metabolic genes (AMGs) were discovered in the virome of WWTPs, and a large array of AMGs involved in biogeochemical cycles like carbon, sulfur, and phosphorus cycling showed transcriptional activation in wastewater treatment systems. •A tiny proportion (less than 0.08% of viral operational taxonomic units) of viruses was discovered to harbor antibiotic resistance genes (ARGs) (29 phage-born ARGs) only in the cellular fraction, and phage-born ARG types primarily included macrolide–lincosamide–streptogramin, tetracycline, and aminoglycoside resistance.
antibiotic resistance gene / auxiliary metabolic gene / metagenome / virome / virus / wastewater treatment system
2024 The Authors. iMeta published by John Wiley & Sons Australia, Ltd on behalf of iMeta Science.
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