Identification of a DUF538 gene conferring resistance to tea green leafhopper (Empoasca onukii) in Camellia sinensis

Acknowledgements

This work was supported by the Fujian Provincial Natural Science Foundation (2024J01372), the National Key Research and Development Program of China (2024YFD1200504), the Fujian Provincial Public Welfare Project (2021R1029003), the Central Guidance Specialization Project (2023L3026), and Jiangxi Province talent plan 646 (jxsq2023102020).

Authors contributions

M.Y., L.C., and Q.Z. designed this project and coordinated research activities. Y.W., C.M., X.K., and F.A. collected the tea cultivar samples. F.A, and C.M, analyzed the egg numbers on each tea cultivars, Y.W., Y.Z., and Q.Z. analyzed the population re-sequencing data. Q.Z. and Y.W. interpreted the data and contributed to the manuscript writing.

Data availability

The detailed table containing sample information, sequencing depth, and key experimental/ analytical data is available at: https://figshare.com/articles/dataset/Supplementary_of_tableS1_and_method/29937992. Additionally, the raw sequencing data (SRA) has been deposited in the Genome Sequence Archive (GSA) at China National Genomics Data Center (NGDC) under Accession Number: CRA021054.

Conflicts of interest statement

The authors declare no conflicts of interest.

References

[1] Zhao Q, Shi L, He W. et al. Genomic variations in the tea leafhop-per reveal the basis of its adaptive evolution. Genom Proteom Bioinform. 2022; 20:1092-105

[2] Li H, Gu Z, Li W. et al. Analysis on the registration status and residue limit standards of pesticides used in tea of China. China Tea. 2024; 46:36-47

[3] Gholizadeh A, Kohnehrouz S. DUF 538 protein super family is predicted to be the potential homologue of bactericidal/permeability-increasing protein in plant system. Protein J. 2013; 32:163-71

[4] Zhao X, Chen S, Wang S. et al. Defensive responses of tea plants (Camellia sinensis) against tea green leafhopper attack: a multi-omics study. Front Plant Sci. 2020; 10:1705

[5] Mhoswa L, O’Neill M, Mphahlele M. et al. A genome-wide asso-ciation study for resistance to the insect pest Leptocybe invasa in Eucalyptus grandis reveals genomic regions and positional candidate defense genes. Plant Cell Physiol. 2020; 61:1285-96