Callus-mediated plant regeneration and CRISPR/Cas9-targeted mutagenesis in Oenanthe javanica

Kai Feng , Cheng Yao , Hui Lv , Zhiyuan Yang , Jialu Liu , Ziqi Zhou , Nan Sun , Shuping Zhao , Peng Wu , Aisheng Xiong , Liangjun Li

Horticulture Research ›› 2026, Vol. 13 ›› Issue (2) : 327

PDF (670KB)
Horticulture Research ›› 2026, Vol. 13 ›› Issue (2) :327 DOI: 10.1093/hr/uhaf327
Letters to the Editor
research-article
Callus-mediated plant regeneration and CRISPR/Cas9-targeted mutagenesis in Oenanthe javanica
Author information +
History +
PDF (670KB)

Cite this article

Download citation ▾
Kai Feng, Cheng Yao, Hui Lv, Zhiyuan Yang, Jialu Liu, Ziqi Zhou, Nan Sun, Shuping Zhao, Peng Wu, Aisheng Xiong, Liangjun Li. Callus-mediated plant regeneration and CRISPR/Cas9-targeted mutagenesis in Oenanthe javanica. Horticulture Research, 2026, 13(2): 327 DOI:10.1093/hr/uhaf327

登录浏览全文

4963

注册一个新账户 忘记密码

Acknowledgements

This study was financially supported by the Jiangsu seed industry revitalization project (JBGS[2021]017), China Agriculture Research System (CARS-24), National Natural Science Foundation of China (32102368).

Authors contributions

L.L. and A.X. initiated and designed the research. K.F., C.Y., H.L., Z.Y., J.L., Z.Z., N.S., S.Z., and P.W. performed the experiments. K.F. and Z.Y. analyzed the data. L.L. and A.X. contributed reagents/materials/analysis tools. K.F. wrote the manuscript. L.L. and A.X. revised the manuscript. All authors read and approved the final manuscript.

Data availability

The supplementary methods and data supporting the conclusions of this article are available on the Figshare database under the accession https://doi.org/10.6084/m9.figshare.30617534.v2.

Conflicts of interest statement

The authors declare that there are no competing interests.

References

[1]

Wang XJ, Luo Q, Li T. et al. Origin, evolution, breeding, and omics of Apiaceae: a family of vegetables and medicinal plants. Hortic Res. 2022;9:uhac076

[2]

Lu CL, Li XF. A review of Oenanthe javanica (Blume) DC. as traditional medicinal plant and its therapeutic potential. Evid Based Complement Alternat Med. 2019; 2019:1-17

[3]

He Z, Sheng SY, Wang LQ. et al. Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC). Plant Methods. 2024; 20:6

[4]

Zhang X, Xu G, Cheng C. et al. Establishment of an Agrobacterium-mediated genetic transformation and CRISPR/Cas9-mediated targeted mutagenesis in Hemp (Cannabis sativa L.). Plant Biotechnol J. 2021; 19:1979-87

[5]

Wolabu TW, Mahmood K, Jerez IT. et al. Multiplex CRISPR/Cas9-mediated mutagenesis of alfalfa FLOWERING LOCUS Ta1 (MsFTa1) leads to delayed flowering time with improved forage biomass yield and quality. Plant Biotechnol J. 2023; 21:1383-92

[6]

Feng K, Liu JL, Sun N. et al. Telomere-to-telomere genome assembly reveals insights into the adaptive evolution of herbivore-defense mediated by volatile terpenoids in Oenanthe javanica. Plant Biotechnol J. 2025; 23: 2346-57

[7]

Liu JX, Li T, Wang H. et al. CRISPR/Cas9-mediated precise targeted mutagenesis of phytoene desaturase in celery. Hortic Res. 2022;9:uhac162

[8]

Xu ZS, Feng K, Xiong AS. CRISPR/Cas9-mediated multiply tar-geted mutagenesis in orange and purple carrot plants. Mol Biotechnol. 2019; 61:191-9

[9]

Xie XR, Ma XL, Zhu QL. et al. CRISPR-GE: a convenient soft-ware toolkit for CRISPR-based genome editing. Mol Plant. 2017; 10:1246-9

[10]

Hu N, Xian Z, Li N. et al. Rapid and user-friendly open-source CRISPR/Cas9 system for single- or multi-site editing of tomato genome. Hortic Res. 2019; 6:7

PDF (670KB)

272

Accesses

0

Citation

Detail

Sections
Recommended

/