Population genetics and origin of horticultural germplasm in Clematis via genotyping-by-sequencing

Acknowledgements

This research was funded by Wenzhou Forestry and Flower Breeding Cooperation Group Project (ZX2024004-3), the National Natural Science Foundation of China (32102428), Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (2021C02071-6), and Wenzhou Major Technological Innovation Project (ZS2020002).

Author contributions

Y.H., Y.Z., and J.Z. conceived the project; Y.H. and Q.H. performed the collection and morphological identification; Y.H., Q.H., X.M., and X.Z. conducted the population genetic analyses; Y.H. wrote the manuscript. All authors have revised and approved the manuscript.

Data availability

The high-throughput sequencing data in this study are open access and uploaded to the National Center for Biotechnology Information (NCBI) under accession number PRJNA1094651.

Conflict of interest statement

The authors declare no competing interests.

References

[1] Swarup S, Cargill EJ, Crosby K. et al. Genetic diversity is indis-pensable for plant breeding to improve crops. Crop Sci. 2021;61: 839-52

[2] Salgotra RK, Chauhan BS. Genetic Diversity, Conservation, and Utilization of Plant Genetic Resources. Genes. 2023;14: 174-87

[3] Zhang H, Jiang C, Wang R. et al. Insights into heat response mech-anisms in Clematis species: physiological analysis, expression profiles and function verification. Plant Mol Biol. 2021;106: 569-87

[4] Zhao Z, Zhang H, Wang P. et al. Development of SSR molecular markers and genetic diversity analysis of Clematis acerifolia from Taihang Mountains. PLoS One. 2023;18:e0285754

[5] Li Y, Li S, Li J. et al. Inter-simple sequence repeat (ISSR) markers revealed genetic diversity and population structure among 9 wild species of Clematis L. Journal of Biobased Materials and Bioen-ergy. 2021;15: 580-8

[6] Yuan T, Wang LY, Roh MS. Confirmation of Clematis hybrids using molecular markers. Scientia Horticulturae. 2010;125: 136-145

[7] Wang N, Yuan Y, Wang H. et al. Applications of genotyping-by-sequencing (GBS) in maize genetics and breeding. Sci Rep. 2020;10:16308

[8] Rodionov A, Amosova AV, Belyakov EA. et al. Genetic conse-quences of interspecific hybridization, its role in speciation and phenotypic diversity of plants. Russ J Genet. 2019;55: 278-94

[9] Payseur BA, Rieseberg LH. A genomic perspective on hybridiza-tion and speciation. Mol Ecol. 2016;25: 2337-60

[10] Goulet BE, Roda F, Hopkins R. Hybridization in plants: old ideas, new techniques. Plant Physiol. 2017;173: 65-78