Circadian rhythms of microbial communities and their role in regulating nitrogen and phosphorus cycling in the rhizosphere of tea plants

Abstract

The circadian clock mediates metabolic functions of plants and rhythmically shapes structure and function of microbial communities in the rhizosphere. However, it is unclear how the circadian rhythm of plant hosts regulates changes in rhizosphere bacterial and fungal communities and nutrient cycles. In the present study, we measured diel changes in the rhizosphere of bacterial and fungal communities, and in nitrogen (N) and phosphorus (P) cycling in 20-year-old tea plantations. The fungal communities were more stable in their responses to circadian changes than bacterial communities in the rhizosphere of the cultivars LJ43 and ZC108. Nevertheless, fungal genera with circadian rhythms were more numerous and had a higher abundance at midnight. Organic P and N mineralization in the rhizosphere was more intensive in LJ43 under day-night alterations, while inorganic N and P cycling was more easily affected by circadian rhythms in ZC108. The rhizosphere denitrification encoded by the genes AOA and AOB was intensive in the morning, irrespective of tea cultivar. Genes related to rhizosphere N fixation (nifH) and denitrification (nosZ and nirK) expressed at greater levels in ZC108, and they reached a peak at midnight. Moreover, the diel rhythm of rhizosphere microbial communities in ZC108 largely regulated dial changes in N and P cycling. These results suggested that the bacterial and fungal communities in the rhizosphere respond differently to circadian rhythms, and they vary between tea cultivars. The timing of bacterial and fungal cycling largely regulates rhizosphere N and P cycling and their ecological functions.

Acknowledgements

The authors wish to thank Dr. Yingtao Sun (Guangzhou Institute of Geochemistry, Chinese Academy of Sciences) for assistance in data processing. This work was supported by the Talent Program of the Zhejiang University (0022112).

Author contributions

M.L. had the main responsibility for data collection, analysis, and writing. J.W. contributed to data collection. Z.L. contributed to the interpretation of data. X.L. and H.K. contributed to manuscript writing and revision. C.L. (the corresponding author) had the overall responsibility for experimental design and project management.

Data availability statement

Raw DNA sequence files and associated metadata were deposited in the NCBI data bank with the accession number PRJNA1010133. All records of statistical analyses are included in Supplementary Materials2. They were created with the SPSS software (version 22).

Conflict of interests

The authors declare that they have no conflict of interest.

Supplementary information

Supplementary data is available at Horticulture Research online.

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