Protein subcellular localization and functional studies in horticultural research: problems, solutions, and new approaches

Acknowledgements

This work was supported by the National Key Research and Development Program (2019YFD1000103), NSFC grants (Nos 31772281, 92254307, 91854102), Foundation of Hubei Hongshan Laboratory (2021hszd016) to P.W., and Hubei province grant (2021CFB142) to Z.B.

Data availability

The data used to support the findings of this study are available from the corresponding author upon request.

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary data

Supplementary data is available at Horticulture Research online.

References

[1] Kang BH, Anderson CT, Arimura S et al. A glossary of plant cell structures: Current insights and future questions. Plant Cell. 2022; 34: 10-52.

[2] Nelson BK, Cai X, Nebenfuhr A . A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants . Plant J. 2007; 51: 1126-36.

[3] Cui Y, Zhao Q, Hu S et al. Vacuole biogenesis in plants: how many vacuoles, how many models? Trends Plant Sci. 2022; 25: 538-48.

[4] Wu S, Zhang BY, Keyhaninejad N et al. A common genetic mechanism underlies morphological diversity in fruits and other plant organs. Nat Commun. 2018; 9: 4734.

[5] Li YF, Deng M, Liu HF et al. ABNORMAL SHOOT 6 interacts with KATANIN 1 and SHADE AVOIDANCE 4 to promote cortical microtubule severing and ordering in Arabidopsis . J Integr Plant Biol. 2021; 63: 646-61.

[6] Smertenko AP, Chang HY, Wagner V et al. The Arabidopsis microtubule-associated protein AtMAP65-1: Molecular analysis of its microtubule bundling activity. Plant Cell. 2004; 16: 2035-47.

[7] Menges M, Samland AK, Planchais S et al. The D-type cyclin CYCD3;1 is limiting for the G1-to-S-phase transition in Arabidopsis . Plant Cell. 2006; 18: 893-906.

[8] Xu J, Lee YJ, Liu B . Establishment of a mitotic model system by transient expression of the D-type cyclin in differentiated leaf cells of tobacco (Nicotiana benthamiana) . New Phytol. 2020; 226: 1213-20.

[9] Yamaguchi M, Kubo M, Fukuda H et al. Vascular-related NAC-DOMAIN7 is involved in the differentiation of all types of xylem vessels in Arabidopsis roots and shoots. Plant J. 2008; 55: 652-64.

[10] Sparkes IA, Runions J, Kearns A et al. Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants. Nat Protoc. 2006; 1: 2019-25.

[11] Martin K, Kopperud K, Chakrabarty R et al. Transient expression in Nicotiana benthamiana fluorescent marker lines provides enhanced definition of protein localization, movement and interactions in planta . Plant J. 2009; 59: 150-62.

[12] Wang PW, Hawkins TJ, Richardson C et al. The plant cytoskeleton, NET3C, and VAP27 mediate the link between the plasma membrane and endoplasmic reticulum. Curr Biol. 2014; 24: 1397-405.

[13] Boudaoud A, Burian A, Borowska-Wykret D et al. FibrilTool, an ImageJ plug-in to quantify fibrillar structures in raw microscopy images. Nat Protoc. 2014; 9: 457-63.

[14] Bao Z, Guo Y, Deng Y et al. The microtubule-associated protein SlMAP70 interacts with SlIQD21 and regulates fruit shape formation in tomato. bioRxiv 2022; 2022.08.08.503161, preprint: not peer reviewed.