Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages

Abstract

It has been observed that the consumption of litchi often causes symptoms characterized by itching or sore throat, gum swelling, oral cavity ulcers and even fever and inflammation, which significantly impair the quality of life of a large population. Using the RAW264.7 cell line, a step-by-step strategy was used to screen for the components in litchi fruits that elicited adverse reactions. The adverse reaction fractions were identified by mass spectrometry and analyzed using the SMART program, and a sequence alignment of the homologous proteins was performed. MTT tests were used to determine the cytotoxicity of a litchi protein extract in RAW264.7 macrophages, and real-time PCR was applied to analyze the expression of inflammatory genes in the RAW264.7 cells treated with lipopolysaccharide or the litchi protein extract. The results showed that the litchi water-soluble protein extract could increase the production of the pro-inflammatory mediators IL-1β, iNOS and COX-2, and the anti-inflammatory mediator HO-1 in the RAW264.7 cell line. The 14-3-3-like proteins GF14 lambda, GF14 omega and GF14 upsilon were likely the candidate proteins that caused the adverse effects.

References

[1] Sicherer SH, Leung DYM . Advances in allergic skin disease, anaphylaxis, and hypersensitivity reactions to foods, drugs, and insects in 2012. J Allergy Clin Immunol 2013; 131: 55-66.

[2] HOM J . An Illustration of Chinese Oral Health Beliefs through shang huo 2007.

[3] Huang CJ, Wu MC . Differential effects of foods traditionally regarded as ‘heating’ and ‘cooling’ on prostaglandin E-2 production by a macrophage cell line. J Biomed Sci 2002; 9: 596-606.

[4] Yoon T, Lee DY, Lee AY, Choi G, Choo BK, Kim HK . Anti-inflammatory effects of Glehnia littoralis extract in acute and chronic cutaneous inflammation. Immunopharmacol Immunotoxicol 2010; 32: 663-670.

[5] Huang ZJ, Zheng GH, Tao JY, Ruan JH . Anti-inflammatory effects and mechanisms of usnic acid. J Wuhan Univ Technol Mater Sci Ed 2011; 26: 955-959.

[6] Pengal RA, Ganesan LP, Wei G, Fang HQ, Ostrowski MC, Tridandapani S . Lipopolysaccharide-induced production of interleukin-10 is promoted by the serine/threonine kinase Akt. Mol Immunol 2006; 43: 1557-1564.

[7] Yan HQ, Ji Q, Chen DD, Wu JL, Peng S, Ma ZC et al. A novel macromolecular extract screened from satsuma with pro-inflammatory effect. Food Funct 2014; 5: 295-302.

[8] Chao CY, Sung PJ, Wang WH, Kuo YH . Anti-inflammatory effect of momordica charantia in sepsis mice. Molecules 2014; 19: 12777-12788.

[9] Ma Q, Xie HH, Li S, Zhang RF, Zhang MW, Wei XY . Flavonoids from the Pericarps of Litchi chinensis. J Agric Food Chem 2014; 62: 1073-1078.

[10] Wall MM . Ascorbic acid and mineral composition of longan (Dimocarpus longan), lychee (Litchi chinensis) and rambuan (Nephelium lappaceum) cultivars grown in Hawaii. J Food Compos Anal 2006; 19: 655-663.

[11] Su DX, Ti HH, Zhang RF, Zhang MW, Wei ZC, Deng YY et al. Structural elucidation and cellular antioxidant activity evaluation of major antioxidant phenolics in lychee pulp. Food Chem 2014; 158: 385-391.

[12] Hoppe S, Steinhart H, Paschke A . Identification of a 28 kDa lychee allergen as a triose-phosphate isomerase. Food Agric Immunol 2006; 17: 9-19.

[13] Raap U, Schaefer T, Kapp A, Wedi B . Exotic food allergy: Anaphylactic reaction to lychee. J Investig Allergol Clin Immunol 2007; 17: 199-201.

[14] Hoppe S, Neidhart S, Zunker K, Hutasingh P, Carle R, Steinhart H et al. The influences of cultivar and thermal processing on the allergenic potency of lychees (Litchi chinensis SONN.). Food Chem 2006; 96: 209-219.

[15] Fah J, Wuthrich B, Vieths S . Anaphylactic reaction to lychee fruit: evidence for sensitization to profilin. Clin Exp Allergy 1995; 25: 1018-1023.

[16] Xiong GZ, Li L, Sun SW, Li TP, Liao DF, Shu C et al. Subcellular localization of DAXX influence ox-LDL induced apoptosis in macrophages. Mol Biol Rep 2014; 41: 7183-7190.

[17] Ebo DG, Ahrazem O, Lopez-Torrejon G, Bridts CH, Salcedo G, Stevens WJ . Anaphylaxis from mandarin (Citrus reticulata): Identification of potential responsible allergens. Int Arch Allergy Immunol 2007; 144: 39-43.

[18] Livak KJ, Schmittgen TD . Analysis of relative gene expression data using real-time quantitative PCR and the 2-DELTADELTACT method. Methods (Orlando) 2001; 25: 402-408.

[19] Wisniewski JR, Zougman A, Nagaraj N, Mann M . Universal sample preparation method for proteome analysis. Nat Methods 2009; 6: 359-362.

[20] Zuidmeer L, Goldhahn K, Rona RJ, Gislason D, Madsen C, Summers C et al. The prevalence of plant food allergies: a systematic review. J Allergy Clin Immunol 2008; 121: 1210-1218.

[21] Roehr CC, Edenharter G, Reimann S, Ehlers I, Worm M, Zuberbier T et al. Food allergy and non-allergic food hypersensitivity in children and adolescents. Clin Exp Allergy 2004; 34: 1534-1541.

[22] He RR, Tsoi B, Li YF, Yao XS, Kurihara H . The Anti-stress effects of guangdong herbal tea on immunocompromise in mice loaded with restraint stress. J Health Sci 2011; 57: 255-263.

[23] Li W, Yadeta KA, Elmore JM, Coaker G . The Pseudomonas syringae effector HopQ1 promotes bacterial virulence and interacts with tomato 14-3-3 proteins in a phosphorylation-dependent manner. Plant Physiol 2013; 161: 2062-2074.

[24] Li HL, Liu DQ, He H, Zhang NN, Ge F, Chen CY . Molecular cloning of a 14-3-3 protein gene from Lilium regale Wilson and overexpression of this gene in tobacco increased resistance to pathogenic fungi. Sci Hortic 2014; 168: 9-16.

[25] Chang MJ, Tao X, Gu YH, Lin GX, Shao HH, Cao QH et al. Cloning and characterization of the 14-3-3 protein gene from Ipomoea batatas (L.) Lam. Afr J Microbiol Res 2012; 6: 1990-1999.

[26] Kilani RT, Maksymowych WP, Aitken A, Boire G, St-Pierre Y, Li Y et al. Detection of high levels of 2 specific isoforms of 14-3-3 proteins in synovial fluid from patients with joint inflammation. J Rheumatol 2007; 34: 1650-1657.

[27] Powell DW, Rane MJ, Joughin BA, Kalmukova R, Hong JH, Tidor B et al. Proteomic identification of 14-3-3ζ as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding. Mol Cell Biol 2003; 23: 5376-5387.

[28] Zhao GY, Ding JY, Gu J, Lu CL, Lin ZW, Guo J et al. The overexpression of 14-3-3 zeta and Hsp27 promotes non-small cell lung cancer progression. Cancer 2014; 120: 652-663.

[29] Liu TA, Jan YJ, Ko BS, Liang SM, Chen SC, Wang J et al. 14-3-3 epsilon overexpression contributes to epithelial-mesenchymal transition of hepatocellular carcinoma. PLos One 2013; 8: e57968.

[30] Liou JY, Ghelani D, Yeh S, Wu KK . Nonsteroidal anti-inflammatory drugs induce colorectal cancer cell apoptosis by suppressing 14-3-3 epsilon. Cancer Res 2007; 67: 3185-3191.

[31] Vercoutter-Edouart AS, Lemoine J, Le Bourhis X, Louis H, Boilly B, Nurcombe B et al. Proteomic analysis reveals that 14-3-3σ is down-regulated in human breast cancer cells. Cancer Res 2001; 61: 76-80.

[32] Sehnke PC, DeLille JM, Ferl RJ . Consummating signal transduction: the role of 14-3-3 proteins in the completion of signal-induced transitions in protein activity. Plant Cell 2002; 14: S339-S354.

[33] Ling SB, Feng TT, Jia KQ, Tian Y, Li Y . Inflammation to cancer: The molecular biology in the pancreas (Review). Oncol Lett 2014; 7: 1747-1754.

[34] Barber AE, Coyle SM, Fischer E, Smith C, van der Poll T, Shires GT et al. Influence of hypercortisolemia on soluble tumor necrosis factor receptor II and interleukin-1 receptor antagonist responses to endotoxin in human beings. Surgery 1995; 118: 406-411.

[35] Tilg H, Trehu E, Atkins MB, Dinarello CA, Mier JW . Interleukin-6 (IL-6) as an anti-inflammatory cytokine: induction of circulating IL-1 receptor antagonist and soluble tumor necrosis factor receptor p55. Blood 1994; 83: 113-118.

[36] Serhan CN, Savill J . Resolution of inflammation: The beginning programs the end. Nat Immunol 2005; 6: 1191-1197.

[37] Andreasen AS, Krabbe KS, Krogh-Madsen R, Taudorf S, Pedersen BK, Moller K . Human Endotoxemia as a model of systemic inflammation. Curr Med Chem 2008; 15: 1697-1705.