Assessing temporal flux of plant hormones in stored processing potatoes using high definition accurate mass spectrometry

Abstract

Plant hormones are important molecules which at low concentration can regulate various physiological processes. Mass spectrometry has become a powerful technique for the quantification of multiple classes of plant hormones because of its high sensitivity and selectivity. We developed a new ultrahigh pressure liquid chromatography–full-scan high-definition accurate mass spectrometry method, for simultaneous determination of abscisic acid and four metabolites phaseic acid, dihydrophaseic acid, 79-hydroxy-abscisic acid and abscisic acid glucose ester, cytokinins zeatin, zeatin riboside, gibberellins (GA1, GA3, GA4 and GA7) and indole-3-acetyl-L-aspartic acid. We measured the amount of plant hormones in the flesh and skin of two processing potato cvs. Sylvana and Russet Burbank stored for up to 30 weeks at 6 °C under ambient air conditions. Herein, we report for the first time that abscisic acid glucose ester seems to accumulate in the skin of potato tubers throughout storage time. The method achieved a lowest limit of detection of 0.22 ng g⁻¹ of dry weight and a limit of quantification of 0.74 ng g⁻¹ dry weight (zeatin riboside), and was able to recover, detect and quantify a total of 12 plant hormones spiked on flesh and skin of potato tubers. In addition, the mass accuracy for all compounds (<5 ppm) was evaluated.

References

[1] Biemelt S, Hajirezaei M, Hentschel E, Sonnewald U . Comparative analysis of abscisic acid content and starch degradation during storage of tubers harvested from different potato varieties. Potato Res 2000; 43: 371-382.

[2] Suttle JC . Physiological regulation of potato tuber dormancy. Am J Potato Res 2004; 81: 253-262.

[3] Suttle JC, Banowetz GM . Changes in cis-zeatin and cis-zeatin riboside levels and biological activity during potato tuber dormancy. Physiol Plantarum 2000; 109: 68-74.

[4] Hartmann A, Senning M, Hedden P, Sonnewald U, Sonnewald S . Reactivation of meristem activity and sprout growth in potato tubers require both cytokinins and gibberellin. Plant Physiol 2011; 155: 776-796.

[5] Rentzsch S, Podzimska D, Voegele A et al. Dose- and tissue-specific interaction of monoterpenes with the gibberellin-mediated release of potato tuber bud dormancy, sprout growth and induction of a-amylases and b-amylases. Planta 2012; 235: 137-151.

[6] Turnbull CGN, Hanke DE . The control of bud dormancy in potato tubers. Measurement of the seasonal pattern of changing concentrations of zeatin-cytokinins. Planta 1985; 165: 366-376.

[7] Izumi Y, Okazawa A, Bamba T, Kobayashi A, Fukusaki E . Development of a method for comprehensive and quantitative analysis of plant hormones by highly sensitive nanoflow liquid chromatography-electrospray ionization-ion trap mass spectrometry. Anal Chim Acta 2009; 648: 215-225.

[8] Alarcón Flores MI, Romero-Gonzalez R, Frenich AG, Vidal JLM . Quechers-based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC-MS/MS. J Sep Sci 2011; 34: 1517-1524.

[9] Li Y, Wei F, Dong X, Peng J, Liu S, Chen H . Simultaneous analysis of multiple endogenous plant hormones in leaf tissue of oilseed rape by solid-phase extraction coupled with high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. Phytochem Anal 2011; 22: 442-449.

[10] Chiwocha SDS, Abrams SR, Ambrose SJ et al. A method for profiling classes of plant hormones and their metabolites using liquid chromatography-electrospray ionization tandem mass spectrometry: an analysis of hormones regulation or thermodormancy of lettuce (Lactuca sativa L.) seeds. Plant J 2003; 35: 405-417.

[11] Pan X, Welti R, Wang X . Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography-electrospray tandem mass spectrometry. Phytochemistry 2008; 69: 1773-1781.

[12] Foukaraki SG, Chope GA, Terry LA . Ethylene exposure after dormancy break is as effective as continuous ethylene to control sprout growth in some UK-grown potato cultivars. Acta Hort 2012; 934: 1175-1181.

[13] Foukaraki SG, Chope GA, Terry LA . 1-MCP Application before continuous ethylene storage supresseus sugar accumulation in the UK-grown potato cultivar ‘Marfona’. Acta Hort 2012; 945: 291-296.

[14] Foukaraki SG, Cools K, Chope GA, Terry LA . Effect of the transition between ethylene and air storage on postharvest quality in six UK-grown potato cultivars. J Hort Sci Biotech 2014; 89: 599-606.

[15] Suttle JC . Postharvest changes in endogenous ABA levels and ABA metabolism in relation to dormancy in potato tubers. Physiol Plantarum 1995; 95: 233-240.

[16] Destefano-Beltran L, Kauber D, Huckle L, Suttle JC . Effects of postharvest storage and dormancy status on ABA content, metabolism and expression of genes involved in ABA biosynthesis and metabolism in potato tuber tissues. Plant Mol Biol 2006; 61: 687-697.

[17] Lulai EC, Suttle JC, Pederson SM . Regulatory involvement of abscisic acid in potato tuber wound-healing. J Exp Bot 2008; 59: 1175-1186.

[18] Krochko JE, Abrams GD, Loewen MK, Abrams SR, Cutler AJ . (+)-abscisic acid 8′-hydroxylase is a cytochrome P450 monooxygenase. Plant Physiol 1998; 118: 849-860.

[19] Saito S, Hirai N, Matsumoto C et al. Arabidopsis CYP707As encode (+)-Abscisic acid 8′-Hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid. Plant Physiol 2004; 134: 1439-1449.

[20] Suttle JC, Lulai EC, Huckle LL, Neubauer JD . Wounding of potato tubers induce increases in ABA biosynthesis and catabolism and alters expression of ABA metabolic genes. J Plant Physiol 2013; 170: 560-566.

[21] Boyer GL, Zeevaart JAD . Isolation and quantitation of b-D-glucopyranosyl abscisate from leaves of xanthium and spinach. Plant Physiol 1982; 70: 227-231.

[22] Hocher V, Sotta B, Maldiney R, Miginiac E . Changes in abscisic acid and its b-D-glucopyranosyl ester levels during tomato (Lycopersicon esculentum Mill.) seed development. Plant Cell Rep 1991; 10: 444-447.

[23] Bray EA, Zeevaart JAD . The compartmentation of abscisic acid and b-D-glucopyranosyl abscisate in mesophyll cells. Plant Physiol 1985; 79: 719-722.

[24] Sauter A, Hartung W . The contribution of internode and mesocotyl tissues to root-to-shoot signalling of abscisic acid. J Exp Bot 2002; 53: 297-302.

[25] Wilkinson S, Davies WJ . ABA-based chemical signalling: the co-ordination of responses to stress in plants. Plant Cell Environ 2002; 25: 195-210.

[26] Nambara E, Marion-Poll A . Abscisic acid biosynthesis and catabolism. Annu Rev Plant Biol 2005; 56: 165-185.