Aqueous extract of Corchorus olitorius decreases cytotoxicity of aflatoxin B₁ and fumonisin B₁ in H4IIE-luc cells

Abstract

Aim: Aflatoxin B₁ (AFB₁) and fumonisin B₁ (FB₁) are important food-borne mycotoxins. Co-contamination of foodstuffs with these two mycotoxins is well-known and has been implicated in a possible development of hepatocellular carcinoma in humans living in regions of the world where exposures to these mycotoxins in grain are greatest. The aim of the current study was to evaluate the potential protective effects of an aqueous extract of Cochorus olitorius (C. olitorius, moroheiya) against cytotoxicity of AFB₁ and/or FB₁ in H4IIE-luc rat hepatoma cells, using assays to measure cell viability and disruption of DNA integrity. Although this transactivation assay was originally developed to specifically respond to aryl hydrocarbon agonists, this cell line was used because of its hepatic origin.

Methods: H4IIE-luc cells were incubated with different concentrations of AFB₁ and/or FB₁ for 24 and 48 h with or without aqueous extract of C. olitorius.

Results: Both mycotoxins decreased cell viability and increased DNA damage. Cytotoxicity was more pronounced when cells were exposed simultaneously to AFB₁ and FB₁.

Conclusion: Aqueous extract of C. olitorius protected cells against cytotoxicity of mycotoxins. C. olitorius contains a water-soluble, natural chemo-preventative agent for cancer that should be isolated and identified.

Keywords

Anticancer / cytotoxicity / DNA / liver / moroheiya / mycotoxins

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