Fermentative production of dextran using Leuconostoc spp. isolated from fermented food products

Abstract

Leuconostoc spp. (LS1and LI1) isolated from sauerkraut and idli batter was selected for dextran production. To enhance the yield of dextran, effects of various parameters such as sucrose concentration, pH, temperature, incubation and inoculum percentage were analyzed. The optimum sucrose concentration for the Leuconostoc spp. (LS1 and LI1) was found to be 15% and 25% respectively. Isolates produced maximum dextran after 20 h of incubation at 29°C and the optimum pH was found between 8 and 8.5. The inoculum concentration of 7.5% was more favorable for the production of dextran by Leuconostoc spp. (LS1 and LI1). The growth kinetic parameters were studied and compared for the strains LS1 and LI1. Mass production of dextran was carried out using a stirred tank batch reactor. FTIR analysis was done to determine the functional groups of dextran. sephadex is prepared by cross linking dextran using epichlorohydrin and the functional groups are determined by FTIR analysis.

Keywords

dextran / Leuconostoc sp. / FTIR / bio-polymer / sephadex / epichlorohydrin

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Acknowledgements

We are greatly indebted to Vellore Institute of Technology for the constant encouragement, help and support for extending necessary facilities.

Compliance with ethics guidelines

Subathra, Shantan, Mohanasrinivasan declare that they have no conflict of interest.

This article does not contain any studies with human or animal subjects performed by any of the authors.

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg