Effects of plant growth regulators on the rapid proliferation of shoots and root induction in the Chinese traditional medicinal plant Atractylodes macrocephala

Abstract

We developed an efficient plant regeneration protocol for rapidly propagating Atractylodes macrocephala Koidz, an important traditional Chinese medicinal plant, via shoot organogenesis. Shoot multiplication was induced on Murashige-Skoog (MS) medium supplemented with various concentrations of N-phenyl-N-1,2,3-thidiazol-5-ylurea (TDZ), 6-benzylaminopurine (BA) and α-naphthaleneacetic acid (NAA). Rooting was induced on half-strength MS medium supplemented with NAA and indolebutyric acid (IBA). The maximum mean number of shoots (5.61) was obtained from a single explant by the combined effect of 1.08 μmol/L NAA and 2.25 μmol/L TDZ. The longest roots and a minimum number of roots were produced when they were cultured in a medium without plant growth regulators. The shortest roots and the largest number of roots were observed in the medium supplemented with 2.7 μmol/L NAA.

Keywords

Chinese traditional medicinal plant / Atractylodes macrocephala Koidz / multiple shoot / plant regeneration

References

[1] Bedir E, Lata H, Schaneberg B, Khan I A, Moraes R M (2003). Micropropagation of HydrastisCanadensis: Goldenseal a North American Endangered Species. Planta Medica, 69(1): 86-88

[2] Caglar C, Caglar S, Ergin O, Yarim M (2005). The influence of growth regulators on shoot proliferation and rooting of in vitro propagated caper. Journal of Environmental Biology, 26(3): 479-485

[3] Fiola J A, Hassan M A, Swartz H J, Bors R H, McNicols R (1990). Effect of thidiazuron, light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves. Plant Cell Tissue and Organ Culture, 20(3): 223-228

[4] He S T, He F Z, Wu C, Li S X, Liu W X, Yang Y F, Jiang S S, He G (2001). Treatment of rotaviral gastroenteritis with Qiwei Baizhu powder. World Journal of Gastroenterology, 7(5): 735-740

[5] Huang H L, Chen C C, Yeh C Y, Huang R L (2005). Reactive oxygen species mediation of Baizhu-induced apoptosis in human leukemia cells. Journal of Ethnopharmacology, 97(1): 21-29

[6] Kim S H, Jung H N, Lee K Y, Lim J, Lee J C, Jang Y S (2005). Suppression of Th2-type immune response-mediated allergic diarrhea following oral administration of traditional Korean medicine: Atractylodes macrocephala Koidz. Immunopharmacology and Immunotoxicology, 27(2): 331-343

[7] Li C, He L (2006). Establishment of the model of white blood cell membrane chromatography and screening of antagonizing TLR4 receptor component from Atractylodes macrocephala Koidz. Science in China Series C: Life Science, 49(2): 182-189

[8] Liu Y, Ye F, Qiu G. Q, Zhang M, Wang R, He Q Y, Cai Y (2005). Effects of lactone I from Atractylodes macrocephala Koidz on cytokines and proteolysis-inducing factors in cachectic cancer patients. Diyijunyidaxue Xuebao, 25(10): 1308-1311 (in Chinese)

[9] Malik K A, Saxena P K (1992). Regeneration inPhaseolus vulgaris L.: High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron. Planta, 186: 384-389

[10] Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiology Plant, 15: 473-497

[11] Ramakrishnan K, Gnanam R, Sivakumar P, Manickam A (2005). In vitro somatic embryogenesis from cell suspension cultures of cowpea [Vigna unguiculata (L.) Walp]. Plant Cell Reports, 24(8): 449-461

[12] Tang Q Y, Feng M G (1997). Practical Statistics and DPS Data Processing System. Beijing: China Agricultural Press, 171-186 (in Chinese)

[13] Visse C, Qureshi J A, Gill R, Saxena P K (1992). Morphoregulatory role of thidiazuron substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures. Plant Physiology, 99(4): 1704-1707

[14] Zhang Y Q, Xu S B, Lin Y C, Li Q, Zhang X, Lai Y R (2000). Antagonistic effects of 3 sesquiterpene lactones from Atractylodes macrocephalaKoidz on rat uterine contractionin vitro. Acta Pharmacologica Sinica, 21(1): 91-96

[15] Zhu Y Q, Xia G H, Fang H G, Fu S H, He F J (2006). Study on tissue culture and rapid propagation of Atractylodes macrocephala. Zhongyaocai, 29(3): 212-213 (in Chinese)

[16] Peng H S, Wang D Q (2004). Formation and variation about typical medical materials of Baizhu. China Journal of Chinese Materia Medica,29(12): 1133-1135 (in Chinese)

RIGHTS & PERMISSIONS

Higher Education Press and Springer-Verlag Berlin Heidelberg