Seasonal dynamic changes in bacterial compositions in the Inner Mongolia desert steppe

Abstract

Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique was employed to examine the seasonal dynamic changes in bacterial community composition in the Inner Mongolia desert steppe using specific primers F954 and R1369. Bright and reproducible bands were sequenced, and the phylogenic tree was constructed. The results show that the bacterial community composition changed between different seasons. The specific bands were different between the sampling sites with light and heavy levels of degraded grassland. Three main types of bacteria constituting the microbial community in the Inner Mongolia desert steppe belonged to the α, γ and δ-sub phyla of Proteobacteria, Bacteroidetes and Acidobacteria. The unculturable bacteria accounted for 69% of the whole bacterial community of the Inner Mongolia desert steppe.

Keywords

Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) / Inner Mongolia desert steppe / community composition / seasonal dynamic change

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Acknowledgements

The authors are very grateful to Dr. Shubiao Wu from the University of New England, Australia for his kind suggestions to this paper. This work was supported by the National Natural Science Foundation of China (Grant No. 30560030), and the University Scientific Research Foundation of Inner Mongolia (No. NJ05099).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg