Phenotypical and structural characterization of the mutant involved in shoot apical meristem

Abstract

An Arabidopsis mutant induced by T-DNA insertion was studied with respect to its phenotype, microstructure of shoot apical meristem (SAM) and histo-chemical localization of the GUS gene in comparison with the wild type. Phenotypical observation found that the mutant exhibited a dwarf phenotype with smaller organs (such as smaller leaves, shorter petioles), and slower development and flowering time compared to the wild type. Optical microscopic analysis of the mutant showed that it had a smaller and more flattened SAM, with reduced cell layers and a shortened distance between two leaf primordia compared with the wild type. In addition, analysis of the histo-chemical localization of the GUS gene revealed that it was specifically expressed in the SAM and the vascular tissue of the mutant, which suggests that the gene trapped by T-DNA may function in the SAM, and T-DNA insertion could influence the functional activity of the related gene in the mutant, leading to alterations in the SAM and a series of phenotypes in the mutant.

Keywords

Arabidopsis / shoot apical meristem / mutant / GUS / T-DNA