Apoptosis induced by 3,7-dinitrodibenzobromonium salts in K cells

Abstract

We evaluated the inhibitory effect of 3,7-dinitrodibenzobromonium salts (cBr) on the proliferation of human chronic myelogenous leukemia K₅₆₂ cell by trypan blue exclusion test and MTT colorimetric assay. The degree of DNA damage in K₅₆₂ cells treated with cBr, was detected by isotopic tracer method (³H-TdR). The morphological changes of these K₅₆₂ cells were examined by fluorescence and electron microscopy. Biochemical characteristics of K₅₆₂ cells were detected by flow cytometry and ³H-thymidine incorporation assay. Findings indicated that cBr could significantly inhibit cell proliferation and result in DNA damage of K₅₆₂ cells. cBr is a new type of immunostimulant and can induce cell apoptosis.

Keywords

3, 7-dinitrodibenzobromonium salts (cBr) / anti-tumor activity, apoptosis