Mapping the transcription repressive domain in the highly conserved human gene

Abstract

HNULP1, a new member of the basic helix-loop-helix transcription factors, contains a DUF654 domain in its C-terminus and is highly conserved from Drosophilae, yeast, zebrafish to mouse. The function of this motif, however, is currently unknown. In this research, we fused five deletion fragments of the DUF654 domain to the GAL4 DNA-binding domain and then co-transfected with plasmids L8G5-Luc and VP-16. The analysis of the GAL4 luciferase reporter gene indicated that fragments from 228 to 407 amino acids in the DUF654 domain had a strong transcription repression activity. Therefore, this study lays a solid foundation for research on the mechanism of hnulp1 transcriptional regulation and the function of the DUF654 domain.

References

1. Cai Z Y Wang Y Q Yu W S Xiao J Li Y Q Liu L Zhu C B Tan K R Deng Y Yuan W Z Liu M Y Wu X S 2006 hnulp1, abasic helix-loop-helix protein with a novel transcriptional repressivedomain, inhibits transcriptional activity of serum response factorBiochem Biophys Res Commun343973981
2. Goodstadt T L Ponting C P 2001 CHROMA: consensus-basedcolouring of multiple alignments for publicationBioinformatics17845846. doi:10.1093/bioinformatics/17.9.845
3. Liu F Zhu C B Xiao J Wang Y Q Tang W X Yuan W Z Zhao Y L Li Y Q Xiang Z M Wu X S Liu M Y 2005 A novel human KRAB-containing zinc-finger gene ZNF446 inhibitstranscriptional activities of SRE and AP-1Biochem Biophys Res Commun333513. doi:10.1016/j.bbrc.2005.05.069
4. Murre C Mccaw S S Baltimore D 1989 A new DNA binding and dimerization motifin immunoglobulin enhancer binding, daughterless, MyoD, and myc proteinsCell56777783. doi:10.1016/0092‐8674(89)90682‐X
5. Olsson M Durbeej M Ekblom P Hjalt T 2002 Nulp1, a novel basic helix-loop-helix protein expressed broadly duringearly embryonic organogenesis and prominently in developing dorsalroot gangliaCell Tissue Res308361370. doi:10.1007/s00441‐002‐0544‐9
6. Thompson J D Higgins D G Gibson T J 1994 CLUSTAL W: improving the sensitivity ofprogressive multiple sequence alignment through sequence weightingNucleic Acids Res2246734680. doi:10.1093/nar/22.22.4673