Effects of special brain area regional cerebral blood flow abnormal perfusion on learning and memory function and its molecular mechanism in rats

KONG Lingbin1, YANG Zhiyin2, DING Shouhua2, AN Rui3

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PDF(164 KB)
Front. Biol. ›› 2008, Vol. 3 ›› Issue (2) : 147-153. DOI: 10.1007/s11515-008-0033-y

Effects of special brain area regional cerebral blood flow abnormal perfusion on learning and memory function and its molecular mechanism in rats

  • KONG Lingbin1, YANG Zhiyin2, DING Shouhua2, AN Rui3
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Abstract

To study the effect of special brain area regional cerebral blood flow (rCBF) abnormal perfusion on learning and memory function and its molecular mechanism, 64 adult male healthy Sprague-Dawley (SD) rats were randomly divided into two groups, the false operation group (control group) and the operation group (model group). After surgical operation, the operation group undertook bilateral common carotid artery permanent ligation, while the other group did not. Learning and memory function were measured by Y-maze at 4 h, 8 h, 24 h and 3 d after surgical operation, respectively. The rCBF of the right frontal lobe and hippocampus was also detected by the PerifluxPF model laser Doppler flowmetry, and the expressions of c-fos or c-jun or Bcl-2 and Bax were also measured by immune histochemistry S-P method accordingly. Results showed that the rCBF of the right frontal lobe and hippocampus in the operation group was significantly lower than that in the false operation group (P < 0.05). The learning indexes, error number (EN), day of reach standard and total reaction time (TRT) in the operation group, were significantly higher than that in the false operation group (P < 0.05). However, the initiative evasion rate in the operation group was significantly lower than that in the false operation group. The study also found that the rCBF was relatively more, the indexes (EN, the day of reach standard and TRT) relatively fewer, but the initiative evasion rate and the memory keeping rate were relatively more. The positive expression and the average absorbency of Fos and Jun in the operation group were significantly higher than that in the false operation group (P < 0.05). Furthermore, Bax and Bcl-2 positive cells were all increased over time in the operation group, and the expression ratio of Bax/Bcl-2 in the operation group was significantly higher than that in the false operation group (P < 0.01). In conclusion, rCBF decrease can impair the learning and memory function in rats, which may be related to the increase of the expression ratio of c-fos or c-jun or Bcl-2 or Bax in the frontal cortex and hippocampus.

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KONG Lingbin, YANG Zhiyin, DING Shouhua, AN Rui. Effects of special brain area regional cerebral blood flow abnormal perfusion on learning and memory function and its molecular mechanism in rats. Front. Biol., 2008, 3(2): 147‒153 https://doi.org/10.1007/s11515-008-0033-y

References

1. Bao X M Shu S Y 1991 Tridimensionalorientation atlas of ratBeijingPeople's Medical PublishingHouse (in Chinese)
2. Dragunow M Faull R 1989 The use ofc-fos as a metabolic marker in neuronal pathway tracingJ Neurosci Methods29261265. doi:10.1016/0165‐0270(89)90150‐7
3. Gao Y Li J S Liu F 2005 Protective effect of ischemic preconditioningon cerebral ischemia and hypoxia: historical retrospect and researchprogressActa Academiae Medicinae Cpapf4(3)244248 (in Chinese)
4. Gillardon F Lenz C Waschke K F Krajewski S Reed J C Zimmermann M Kuschinsky W 1996 Altered expression of Bcl-2, Bcl-x, Bax and c-Fos colocalizeswith DNA fragmentation and ischemic cell damage following middle cerebralartery occlusion in ratsBrain Res Mol BrainRes40254260. doi:10.1016/0169‐328X(96)00059‐9
5. Han Ju Guo H Z 2004 Effect ofischemic preconditioning on expression of HSP70 and FOS after focalcerebral infarction in ratChinese Journalof Clinical Neurosciences12(2)136139 (in Chinese)
6. Herrera D G Robertson H A 1996 Activationof c-fos in the brainPro Neurobio5083107. doi:10.1016/S0301‐0082(96)00021‐4
7. Krajewski S Mai J K Krajewska M Sikorska M Mossakowski M J Reed J C 1995 Upregulation of bax protein levels inneurons following cerebreal ischemiaJ Naurosci1563466376
8. Li W Zhao H M Yan H J 1995 Establishment of animal model of ischemiclearning memory disorder in miceBasic andClinical Medicine15(6)3640 (in Chinese)
9. Matsuda S Peng H Yoshimura H Wen T C Fukuda T Sakanaka M 1996 Persistent c-fos expression in the brains of mice withchronic social stressNeurosci Res26(2)157170. doi:10.1016/S0168‐0102(96)01088‐7
10. Runyan J D Dash P K 2005 Distinct prefrontalmolecular mechanisms for information storage lasting seconds versusminutesLearn Mem12(3)232238. doi:10.1101/lm.92405
11. Sagar S M Sharp F R Curran T 1988 Expression of c-fos protein in brain:metabolic mapping at the cellular levelScience24013281331. doi:10.1126/science.3131879
12. Takeuchi K Nakamura N Cook N S Pratt R E Dzau V J 1990 Angiotensin II can regulategene expression by the AP-1 binding sequence via a protein kinasec-dependent pathwayBiochem Biophys ResCommun7211891194. doi:10.1016/0006‐291X(90)91574‐C
13. van der Borght K Wallinga A E Luiten PG Eggen B J van der Zee E A 2005 Morris watermaze learning in two rat strains increases the expression of the polysialylatedform of the neural cell adhesion molecule in the dentate gyrus buthas no effect on hippocampal neurogenesisBehav Neurosci119(4)92632. doi:10.1037/0735‐7044.119.4.926
14. Zhou F Guo J C Yang R Gu J Jin H B Wu G C Cheng J S 2006 Effects oftaurine on cerebral blood flow perfusion, cell apoptosis, and infarctvolume in acute cerebral ischemic ratsAdvExp Med Biol583353358
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