Molecular genetic analysis of Dongzhou-period ancient human of Helingeer in Inner Mongolia, China

Abstract

The mtDNA hypervariable region I (HVR-I) of 10 ancient individuals from Dongzhou-period ancient human populations in Helingeer county of Inner Mongolia were amplified and sequenced to investigate the genetic structure. The relationships between the ancient population and related extant populations, as well as its possible origin at the molecular level, were also studied. Moreover, phylogenetic analysis and multidimensional scaling analysis were also performed based on the mtDNA data of the ancient population in Helingeer and the related Eurasian population. The results showed that the ancient population in Helingeer were closer to the northern Asian populations than to the other compared populations in matrilineal lineage. Combining the research results of archaeology and anthropology as well as molecular biology, we inferred that they were nomads who migrated from Mongolia plateau and cis-Baikal region to Helingeer in Inner Mongolia, China.

References

1. Anderson S Bankier A T Barrell B G de Bruijn M H Coulson A R Drouin J Eperon I C Nierlich D P Roe B A Sanger F Schreier P H Smith A J Staden R Young I G 1981 Sequences and organization of the human mitochondrial genomeNature290457465
2. Kings M Stone A Schmitz R W Krainitzki H Stoneking M Pääbo S 1997 Neanderthal DNA sequencesand the origin of modern humansCell901930
3. Kivisild T Tolk H V Parik J Wang Y Papiha S S Bandelt H J Villems R 2002 The emerginglimbs and twigs of the East Asian MtDNA treeMol Biol Evol1917371751
4. Lin Y 2003 The forming orocess of northern nomads belt along the GreatWall of ChinaYenching Journal of ChineseStudies1495145 (in Chinese)
5. Pakendorf B Wiebe V Tarskaia L A Spitsyn V A Soodyall H Rodewald A Stoneking M 2003 MitochondrialDNA evidence for admixed origins of central Siberian populationsAm J Phys Anthropol120211224
6. Ricaut F X Fedoseeva A Keyser-Tracqui C Crubézy E Ludes B 2005 Ancient DNAanalysis of human Neolithic remains found in Northeastern SiberiaAm J Phys Anthropol126458462
7. Schurr T G Sukernik R I Starikovskaya Y B Wallace D C 1999 Mtochondrial DNA variation in Koryaks and Itelmen: populationreplacement in the Okhotsk Sea-Bering Searegion during the NeolithicAm J Phys Anthropol108139
8. Shinoda K Kanai S 1999 Intracemeterygenetic analysis at the Nkazuma Jomon Site in Japan by mitochondrialDNA sequencingAnthropol Sci107129140
9. Xie C Z Liu S B Cui Y Q Zhu H Zhou H 2005 Analysis of mitochondrialDNA from the ancient tombs of ChawuhuJournalof Jilin University (Science Edition)43(4)538540 (in Chinese)
10. Yang S MDingH 2003 Chinese EthnographyBeijingCentralUniversity for Nationalities Press142191 (in Chinese)
11. Zhang X L Cui Y Q Lu H Y Zhou H 2005 Application of phygenetic analysis in ancient DNA researchJournal of Jilin University (Science Edition)43(5)696701 (in Chinese)