Karyotyping of Brassica oleracea L. based on rDNA and Cot-1 DNA fluorescence in situ hybridization

WANG Taixia1, WU Chunhong2, HUANG Jinyong3, WEI Wenhui4

PDF(473 KB)
PDF(473 KB)
Front. Biol. ›› 2007, Vol. 2 ›› Issue (4) : 403-407. DOI: 10.1007/s11515-007-0061-z

Karyotyping of Brassica oleracea L. based on rDNA and Cot-1 DNA fluorescence in situ hybridization

  • WANG Taixia1, WU Chunhong2, HUANG Jinyong3, WEI Wenhui4
Author information +
History +

Abstract

To explore an effective and reliable karyotyping method in Brassica crop plants, Cot-1 DNA was isolated from Brassica oleracea genome, labeled as probe with Biotin-Nick Translation Mix kit, in situ hybridized to mitotic spreads, and where specific fluorescent bands showed on each chromosome pair. 25S and 5S rDNA were labeled as probes with DIG-Nick Translation Mix kit and Biotin-Nick Translation Mix kit, respectively, in situ hybridized to mitotic preparations, where 25S rDNA could be detected on two chromosome pairs and 5S rDNA on only one. Cot-1 DNA contains rDNA and chromosome sites identity between Cot-1 DNA and 25S rDNA was determined by dual-colour fluorescence in situ hybridization. All these showed that the karyotyping technique based on a combination of rDNA and Cot-1 DNA chromosome landmarks is superior to all but one. A more exact karyotype of B. oleracea has been analyzed based on a combination of rDNA sites, Cot-1 DNA fluorescent bands, chromosome lengths and arm ratios.

Cite this article

Download citation ▾
WANG Taixia, WU Chunhong, HUANG Jinyong, WEI Wenhui. Karyotyping of Brassica oleracea L. based on rDNA and Cot-1 DNA fluorescence in situ hybridization. Front. Biol., 2007, 2(4): 403‒407 https://doi.org/10.1007/s11515-007-0061-z
PDF(473 KB)

Accesses

Citations

Detail

Sections
Recommended

/