Optimized cultivation of highly-efficient degradation bacterial strains and their degradation ability towards pyrene

Abstract

Two bacterial strains, Py1 and Py4, have been tamed and isolated through long cultivation with polycyclic aromatic hydrocarbon—pyrene as the single carbon source. It has been proven that they are both highly-efficient pyrene degrading bacteria and both Bacillus sp.. The pyrene degradation ability of separated Py1, Py4 and the consortium of equal Py1 and Py4 was studied in this project. It is shown that pyrene degradation rates were 88% in 10hr by Py1, 84% in 14hr by Py4, and 88% in 8hr by the consortium. It was also determined that the best degradation temperatures were 37vH and pH 7.0 respectively. The influence of different nutrient substrates added in the degradation experiments was also studied. It was shown that sodium salicylate, sodium acetate and yeast extract had obvious simulative effect, but glucose had no obvious effect.