The production-influencing factors of extracellular polysaccharide (EPS) from a strain of lactic acid bacteria and EPS extraction

CHEN Ying; SUN Liping; ZENG Yong; WANG Lei; AN Liguo

doi:10.1007/s11515-006-0028-5

Front. Biol. ›› 2006, Vol. 1 ›› Issue (3) :236 -240. DOI: 10.1007/s11515-006-0028-5

The production-influencing factors of extracellular polysaccharide (EPS) from a strain of lactic acid bacteria and EPS extraction

CHEN Ying¹, SUN Liping², ZENG Yong², WANG Lei³, AN Liguo³

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Abstract

The influencing factors of extracellular polysaccharide (EPS) produced from a strain of lactic acid bacteria (LAB L15) were studied by using the phenol-H₂SO₄ method. It was demonstrated that the strain produced EPS at the most amount when it was incubated for 40 48 h and when the pH value was 4 under 30?C. Glucose was the most suitable carbon source for LAB-producing EPS. The rough EPS was obtained from L15 culture after centrifugation, dialysis, deprotein, decoloration, and ethanol-precipitation. The sample was at least composed of two polysaccharides that were completely different in molecular weight and the amount. The purified EPS was passed through the SephadexG-200 column and it showed that it was a sample purified by thin layer chromatography.

Keywords

lactic acid bacteria, extracellular polysaccharide (EPS), column chromatography, thin layer chromatography

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CHEN Ying, SUN Liping, ZENG Yong, WANG Lei, AN Liguo. The production-influencing factors of extracellular polysaccharide (EPS) from a strain of lactic acid bacteria and EPS extraction. Front. Biol., 2006, 1(3): 236-240 DOI:10.1007/s11515-006-0028-5