An improved method for fluorescence analysis of dissolved organic matter in cave drip water

Xiuli LI, Chaoyong HU, Jin LIAO, Liangliang BAO, Qixi MAO

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PDF(330 KB)
Front. Earth Sci. ›› 2014, Vol. 8 ›› Issue (4) : 595-598. DOI: 10.1007/s11707-014-0439-6
RESEARCH ARTICLE
RESEARCH ARTICLE

An improved method for fluorescence analysis of dissolved organic matter in cave drip water

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Abstract

An improved synchronous fluorimetric method for the determination of dissolved organic matter in cave drip water, by adding ascorbic acid, is described. The method is based on the redox reaction between ascorbic acid and the electron-withdrawing constituents in dissolved organic matter. The results show that adding ascorbic acid can quench the minor peaks, at 200–300 nm, but does not affect the intensity of the main peaks at 300–500 nm. In addition, adding ascorbic acid can maintain relatively high and constant fluorescence intensity over a wide pH range (9–4).

Keywords

drip water / synchronous fluorescence spectroscopy / ascorbic acid / pH

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Xiuli LI, Chaoyong HU, Jin LIAO, Liangliang BAO, Qixi MAO. An improved method for fluorescence analysis of dissolved organic matter in cave drip water. Front. Earth Sci., 2014, 8(4): 595‒598 https://doi.org/10.1007/s11707-014-0439-6

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant Nos. 41072262, 41371216, and 41302145), China Postdoctoral Science Foundation (Grant No. 2013M531757). We are grateful to Prof. Philip A. Meyers and James R. Maxwell for language editing that notably improved the manuscript.

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
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