An improved method for fluorescence analysis of dissolved organic matter in cave drip water
Xiuli LI, Chaoyong HU, Jin LIAO, Liangliang BAO, Qixi MAO
An improved method for fluorescence analysis of dissolved organic matter in cave drip water
An improved synchronous fluorimetric method for the determination of dissolved organic matter in cave drip water, by adding ascorbic acid, is described. The method is based on the redox reaction between ascorbic acid and the electron-withdrawing constituents in dissolved organic matter. The results show that adding ascorbic acid can quench the minor peaks, at 200–300 nm, but does not affect the intensity of the main peaks at 300–500 nm. In addition, adding ascorbic acid can maintain relatively high and constant fluorescence intensity over a wide pH range (9–4).
drip water / synchronous fluorescence spectroscopy / ascorbic acid / pH
[1] |
Arya S P, Mahajan M, Jain P (2000). Non-spectrophotometric methods for the determination of Vitamin C. Anal Chim Acta, 417(1): 1–14
CrossRef
Google scholar
|
[2] |
Baker A (2005). Thermal fluorescence quenching properties of dissolved organic matter. Water Res, 39(18): 4405–4412
CrossRef
Pubmed
Google scholar
|
[3] |
Baker A, Barnes W L, Smart P L (1996). Speleothem luminescence intensity and spectral characteristics: signal calibration and a record of palaeovegetation change. Chem Geol, 130(1–2): 65–76
CrossRef
Google scholar
|
[4] |
Baker A, Genty D (1999). Fluorescence wavelength and intensity variations of cave waters. J Hydrol (Amst), 217(1–2): 19–34
CrossRef
Google scholar
|
[5] |
Ban F, Pan G, Zhu J, Cai B, Tan M (2008). Temporal and spatial variations in the discharge and dissolved organic carbon of drip waters in Beijing Shihua Cave, China. Hydrol Processes, 22(18): 3749–3758
CrossRef
Google scholar
|
[6] |
Blyth A J, Baker A, Collins M J, Penkman K E H, Gilmour M A, Moss J S, Genty D, Drysdale R N (2008). Molecular organic matter in speleothems and its potential as an environmental proxy. Quat Sci Rev, 27(9–10): 905–921
CrossRef
Google scholar
|
[7] |
Coble P G (1996). Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy. Mar Chem, 51(4): 325–346
CrossRef
Google scholar
|
[8] |
Gázquez F, Calaforra J M, Rull F, Forti P, García-Casco A (2012). Organic matter of fossil origin in the amberine speleothems from El Soplao Cave (Cantabria, Northern Spain). International Journal of Speleology, 41(1): 113–123
CrossRef
Google scholar
|
[9] |
Hartland A, Fairchild I J, Lead J R, Baker A (2010). Fluorescent properties of organic carbon in cave dripwaters: effects of filtration, temperature and pH. Sci Total Environ, 408(23): 5940–5950
CrossRef
Pubmed
Google scholar
|
[10] |
Hu C, Henderson G M, Huang J, Chen Z, Johnson K R (2008a). Report of a three-year monitoring programme at Heshang Cave, Central China. International Journal of Speleology, 37(3): 143–151
CrossRef
Google scholar
|
[11] |
Hu C, Henderson G M, Huang J, Xie S, Sun Y, Johnson K R (2008b). Quantification of Holocene Asian monsoon rainfall from spatially separated cave records. Earth Planet Sci Lett, 266(3–4): 221–232
CrossRef
Google scholar
|
[12] |
Hudson N, Baker A, Reynolds D (2007). Fluorescence analysis of dissolved organic matter in natural, waste and polluted waters—A review. River Res Appl, 23(6): 631–649
CrossRef
Google scholar
|
[13] |
McGarry S F, Baker A (2000). Organic acid fluorescence: applications to speleothem palaeoenvironmental reconstruction. Quat Sci Rev, 19(11): 1087–1101
CrossRef
Google scholar
|
[14] |
Miano T M, Martin J P, Sposito G (1988). Fluorescence spectroscopy of humic substances. Soil Sci Soc Am J, 52(4): 1016–1019
CrossRef
Google scholar
|
[15] |
Mobed J, Hemmingsen S L, Autry J L, McGown L B (1996). Fluorescence characterisation of IHSS humic substances: total luminescence spectra with absorbence correction. Environ Sci Technol, 30(10): 3061–3065
CrossRef
Google scholar
|
[16] |
Patel-Sorrentino N, Mounier S, Benaim J Y (2002). Excitation-emission fluorescence matrix to study pH influence on organic matter fluorescence in the Amazon basin rivers. Water Res, 36(10): 2571–2581
CrossRef
Pubmed
Google scholar
|
[17] |
Senesi N, Miano T M, Provenzano M R (1991a). Fluorescence spectroscopy as a means of distinguishing fulvic and humic acids from dissolved and sedimentary aquatic sources and terrestrial sources. Humic Substances in the Aquatic and Terrestrial Environment, 33: 63–73
CrossRef
Google scholar
|
[18] |
Senesi N, Miano T M, Provenzano M R, Brunetti G (1991b). Characterisation, differentiation and classification of humic substances by fluorescence spectroscopy. Soil Sci, 152(4): 259–271
CrossRef
Google scholar
|
[19] |
van Beynen P, Bourbonniere R, Ford D, Schwarcz H (2001). Causes of colour and fluorescence in speleothems. Chem Geol, 175(3–4): 319–341
CrossRef
Google scholar
|
[20] |
Xie S, Yi Y, Huang J, Hu C, Cai Y, Collins M, Baker A (2003). Lipid distribution in a subtropical southern China stalagmite as a record of soil ecosystem response to palaeoclimate change. Quat Res, 60(3): 340–347
CrossRef
Google scholar
|
[21] |
Yang J, Tong C, Jie N, Zhang G, Ren X, Hu J (1997). Fluorescent reaction between ascorbic acid and DAN and its analytical application. Talanta, 44(5): 855–858
CrossRef
Pubmed
Google scholar
|
/
〈 | 〉 |