Abundance and diversity of candidate division JS1- and Chloroflexi-related bacteria in cold seep sediments of the northern South China Sea

Yong ZHANG, Xin SU, Fang CHEN, Lu JIAO, Hongchen JIANG, Hailiang DONG, Guochun DING

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Front. Earth Sci. ›› DOI: 10.1007/s11707-012-0324-0
RESEARCH ARTICLE

Abundance and diversity of candidate division JS1- and Chloroflexi-related bacteria in cold seep sediments of the northern South China Sea

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Abstract

Candidate division JS1-and Chloroflexi-related bacteria are ubiquitous in various deep marine sediments worldwide, yet almost nothing is known about their abundance and diversity in cold seep sediments. Here, we investigated the abundance and diversity of JS1- and Chloroflexi-related bacteria in a cold seep marine sediment core collected from the northern South China Sea (SCS) with the employment of quantitative polymerase chain reaction (qPCR) and 16S rRNA gene phylogenetic analyses. The qPCR results showed that 16S rRNA gene copies per gram of sediments for the total bacteria and JS1- and Chloroflexi-related bacteria were at magnitudes of 108 and 106, respectively. The relative abundance of JS1- and Chloroflexi-related 16S rRNA genes to that of total bacteria was 0.07%–8.78% throughout the core. Phylogenetic analyses showed that the JS-1 related clone sequences were dominant throughout the core. Our study provided insights into abundance and diversity of JS1- and Chloroflexi-related bacteria in the northern SCS cold seep sediments.

Keywords

candidate division JS1 / Chloroflexi / cold seep sediments / South China Sea

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Yong ZHANG, Xin SU, Fang CHEN, Lu JIAO, Hongchen JIANG, Hailiang DONG, Guochun DING. Abundance and diversity of candidate division JS1- and Chloroflexi-related bacteria in cold seep sediments of the northern South China Sea. Front Earth Sci, https://doi.org/10.1007/s11707-012-0324-0

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Acknowledgements

This research was supported by the National Basic Research Program of China (No. 2009CB219502), National Special Foundation (No. GZH200200203-02-01), Non-profit Industry Financial Program of Ministry of Land and Resources of the PRC (No. 200811014-02), and the Fundamental Research Funds for the Central Universities (No. 2-9-2011-197).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
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