Ecology of testate amoebae in Dajiuhu peatland of Shennongjia Mountains, China, in relation to hydrology

Abstract

This study investigates the testate amoeba communities of a large peatland in Central China. The ecology and seasonal variability of testate amoeba communities were studied during 2009–2010. Investigation of environmental controls using ordination showed that the relationship between testate amoeba communities and depth to water table (DWT) and pH are extremely weak. The small proportion of variance explained by water table depth here (only 1.9% in the full data) shows that the hydrological control is weaker than we expected in this peatland, and weaker than any study we are aware of using a similar methodology. Attempts to develop species-environment (transfer function) models or identify indicator species for future palaeoecological studies were unsuccessful. Previous large-scale studies of peatland testate amoeba ecology have been largely restricted to Europe and North America and results have been relatively consistent among studies. Our results contrast with this consensus and suggest that at least in minerotrophic peatlands in China testate amoeba communities may be primarily controlled by different environmental variables. In China, testate amoebae have been relatively little studied but may prove to be valuable for a variety of applications in palaeoecology and biomonitoring and much further work is required.

Keywords

testate amoebae / ecology / minerotrophic / Dajiuhu peatland / China

References

[1] An Z, Stephen C, John E, Wu X, Wang S, Liu X, Li X, Zhou W (2000). Asynchronous Holocene optimum of the East Asian Monsoon. Quat Sci Rev, 19(8): 743-762

[2] Beyens L, Ledeganck P, Graae B J, Nijs I (2009). Are soil biota buffered against climatic extremes? An experimental test on testate amoebae in arctic tundra (Qeqertarsuaq, West Greenland). Polar Biol, 32(3): 453-462

[3] Birks H J B (1995). Quantitative palaeoecological reconstructions. In: Maddy D, Brew S, eds. Statistical Modelling of Quaternary Science Data. Quaternary Research Association, Cambridge, UK

[4] Bobrov A A (2001). Findings of tropical group testate amoebae (Protozoa: Testacea) at the Far East (Sikhote Alin Reserves). Biol Bull, 28: 475-482

[5] Bobrov A A, Charman D J, Warner B G (1999). Ecology of testate amoebae (Protozoa: Rhizopoda) on peatlands in western Russia with special attention to niche separation in closely related taxa. Protist, 150(2): 125-136

[6] Bobrov A A, Mazei Y (2004). Morphological variability of testate amoebae (Rhizopoda: Testacealobosea: Testaceafilosea) in natural populations. Acta Protozool, 43: 133-146

[7] Booth R K (2001). Ecology of testate amoebae (protozoa) in two lake superior coastal wetlands: implications for palaeoecology and environmental monitoring. Wetlands, 21(4): 564-576

[8] Booth R K (2007). Testate amoebae as proxies of mean annual water table depth in Sphagnum-dominated peatlands of North America. J Quaternary Sci, 23(1): 43-57

[9] Booth R K (2011). Testing the climate sensitivity of peat-based paleoclimate reconstructions in mid-continental North America. Quat Sci Rev, 29(5-6): 720-731

[10] Booth R K, Lamentowicz M, Charman D J (2010). Preparation and analysis of testate amoebae in peatland palaeoenvironmental studies. Mires and Peat, 7: 1-7

[11] Booth R K, Zygmunt J R (2005). Biogeography and comparative ecology of testate amoebae inhabiting Sphagnum-dominated peatlands in the Great Lakes and Rocky Mountain regions of North America. Divers Distrib, 11(6): 577-590

[12] Charman D J (1997). Modelling hydrological relationships of testate amoebae (Protozoa: Rhizopoda) on New Zealand peatlands. J R Soc N Z, 27(4): 465-483

[13] Charman D J (2007). Summer water deficit variability controls on peatland water-table changes: implications for Holocene palaeoclimate reconstructions. Holocene, 17(2): 217-227

[14] Charman D J, Barber K E, Blaauw M, Langdon P G, Mauquoy D, Daley T J, Hughes P D M, Karofeld E (2009). Climate drivers for peatland palaeoclimate records. Quat Sci Rev, 28(19-20): 1811-1819

[15] Charman D J, Brown A D, Hendon D, Karofeld E (2004). Testing the relationship between Holocene peatland palaeoclimate reconstructions and instrumental data at two European sites. Quat Sci Rev, 23(1-2): 137-143

[16] Charman D J, Hendon D, Woodland A A (2000). The Identification of testate amoebae (Protozoa: Rhizopoda) from British oligotrophic peats. Quaternary Research Association Technical Guide Series, Cambridge, UK

[17] Clarke K R (1993). Non-parametric multivariate analysis of changes in community structure. Aust J Ecol, 18(1): 117-143

[18] Dufrêne M, Legendre P (1997). Species assemblages and indicator species: The need for a flexible asymmetrical approach. Ecol Monogr, 67: 345-366

[19] Hendon D, Charman D J (1997). The preparation of testate amoebae (Protozoa: Rhizopoda) samples from peat. Holocene, 7(2): 199-205

[20] Juggins S 2003. C2 user guide. Software for ecological and palaeoecological data analysis and visualisation. University of Newcastle, Newcastle upon Tyne, UK

[21] Lamentowicz M, Lamentowicz L, Knaap W O, Gabka M, Mitchell E A (2010). Contrasting species-environment relationships in communities of testate amoebae, bryophytes and vascular plants along the fen-bog gradient. Microb Ecol, 59(3): 499-510

[22] Lamentowicz M, Mitchell E A D (2005). The ecology of testate amoebae (Protists) in Sphagnum in relation to peatland ecology. Microb Ecol, 50: 48-63

[23] Li H K, Bu Z J, Wang S Z, An Z S, Zhao H Y, Ma Y Y, Chen X (2009). Environmental implications of the modern testate amoebae in the peatlands in ChangbaiMountains. Quaternary Science, 29: 817-824 (in Chinese)

[24] Markel E, Booth R K, Qin Y (2010). Testate amoebae and δ¹³C of Sphagnum as surface-moisture proxies in Alaskan peatlands. Holocene, 20(3): 463-475

[25] Meisterfeld R (2002). Order Arcellinida Kent. In: Lee J J, Leedale G F, Bradbury P, eds. An Illustrated Guide to the Protozoa. Lawrence Kansas: Allen Press

[26] Mitchell E A, Bragazza L, Gerdol R (2004). Testate amoebae (Protista) communities in Hylocomium splendens (Hedw.) B.S.G. (Bryophyta): relationships with altitude, and moss elemental chemistry. Protist, 155(4): 423-436

[27] Mitchell E A, Buttler A J, Warner B G, Gobat J M (1999). Ecology of testate amoebae (Protozoa: Rhizopoda) in Sphagnum peatlands in the Jura mountains, Switzerland and France. Ecoscience, 6: 565-576

[28] Mitchell E A, Charman D J, Warner B G (2008). Testate amoebae analysis in ecological and paleoecological studies of wetlands: past, present and future. Biodivers Conserv, 17(9): 2115-2137

[29] Mitchell E A, Gilbert D (2004). Vertical micro-distribution and response to nitrogen deposition of testate amoebae in Sphagnum. J Eukaryot Microbiol, 51(4): 480-490

[30] Ning Y Z, Shen Y F (1999). Community structure and its characteristics of soil protozoa in typical zones of China. Joural of Northwest Normal University, 35: 50-54 (Natural Science)

[31] Payne R (2011). Can testate amoeba-based palaeohydrology be extended to fens? J Quaternary Sci, 26(1): 15-27

[32] Payne R, Charman D J, Matthews S, Eastwood W (2008). Testate amoebae as palaeoclimate proxies in Sürmene Ağaçbaşi Yaylasi peatland (Northeast Turkey). Wetlands, 28(2): 311-323

[33] Payne R, Kishaba K, Blackford J, Mitchell E A (2006). The ecology of testate amoebae in southcentral Alaskan peatlands: building transfer function models for palaeoenvironmental inference. Holocene, 16: 403-414

[34] Payne R, Lamentowicz M, Mitchell E A D (2011). The perils of taxonomic inconsistency inquantitative palaeoecology: experiments with testate amoeba data. Boreas, 40(1): 15-27

[35] Payne R, Mitchell E A(2009). How many is enough? Determining optimal count totals for ecological and palaeoecological studies of testate amoebae. J Paleolimnol, 42(4): 483-495

[36] Payne R, Ryan P A, Nishri A, Gophen M (2010). Testate amoeba communities of the drained Hula wetland (Israel): implications for ecosystem development and conservation management. Wetlands Ecol Manage, 18(2): 177-189

[37] Payne R J, Mitchell E A D (2007). Ecology of testate amoebae from mires in the Central Rhodope Mountains, Greece and development of a transfer function for palaeohydrological reconstruction. Protist, 158(2): 159-171

[38] Penard E (1902). Faune rhizopodique du bassin du Léman. Genève: Henry Kündig

[39] Qin Y, Booth R K, Gu Y, Wang Y, Xie S (2009). Testate amoebae as indicators of 20th century environmental change in Lake Zhangdu, China. Fundamental and Applied Limnology. Arch Hydrobiol, 175: 29-38

[40] Qin Y, Gu Y, Xie S, Zhou X (2007). Recent environmental change in Swan Oxbow of the Yangtze River: evidence from testate amoebae records. Geological Science and Technology Information, 26: 37-42 (in Chinese)

[41] Qin Y, Xie S, Gu Y, Zhou X (2008a). Pontigulasia pangulostoma nov. spec., a new testate amoeba from the peat land of Shennongjia Mountains, China. Acta Protozool, 47: 155-160

[42] Qin Y, Xie S, Smith H G, Swindles G T, Gu Y (2011). Diversity, distribution and biogeography of testate amoebae in China: implications for ecological studies in Asia. Eur J Protistol, 47(1): 1-9

[43] Qin Y, Xie S, Swindles G T, Gu Y, Zhou X (2008b). Pentagonia zhangduensis nov. spec. (Lobosea, Arcellinida), a new freshwater species from China. Eur J Protistol, 44(4): 287-290

[44] Shen Y F 1983. Protozoa of the Teibetan Plateau. In: Jiang X Z, Shen Y F, Gong X J, eds. Aquatic Invertebrates of the Teibetan Plateau. Beijing: Science Press (in Chinese)

[45] Smith H, Coupe S (2002). Testate amoebae - past, present and future. Eur J Protistol, 37(4): 367-369

[46] Swindles G T, Charman D J, Roe H M, Sansum P A (2009). Environmental controls on peatland testate amoebae (Protozoa: Rhizopoda) in the North of Ireland: implications for Holocene palaeoclimate studies. J Paleolimnol, 42(1): 123-140

[47] Woodland W A, Charman D J, Sims P C (1998). Quantitative estimates of water tables and soil moisture in Holocene peatlands from testate amoebae. Holocene, 8(3): 261-273

[48] Yang J, Feng W, Miao W (2004). A taxonomic catalogue of freshwater and soil testacea in China with a discussion of their faunal similarity. Acta Hydrobiologica Sinica, 28: 426-433 (in Chinese)

[49] Yang J, Meisterfeld R, Zhang W J, Shen Y F (2005). Difflugia mulanensis nov. spec., a freshwater testate amoeba from Lake Mulan, China. Eur J Protistol, 41(4): 269-276

[50] Yang J, Shen Y F (2005). Morphology, biometry and distribution of Difflugia biwae Kawamura, 1918 (Protozoa: Rhizopoda). Acta Protozool, 44: 103-111

[51] Zhao Y, Hoelzer A, Yu Z (2007). Late Holocene natural and human-induced environmental change reconstructed from peat records in eastern Central China. Radiocarbon, 49: 789-798

[52] Zhu C, Ma C, Yu S, Tang L, Zhang W, Lu X (2009). A detailed pollen record of vegetation and climate change in Central China during the past 16000 years. Boreas, 38: 69-76

Acknowledgements

This work was supported by the National Basic Research Program of China (No. 2011CB808800), the National Natural Science Foundation of China (Grant Nos. 40930210, 40921062, 40872202, 41072261 and 30800097), the Opening Research Fund of Key Laboratory of Wetland Ecology and Environment (No. WELF-2009-B-003), the Special Fund for Basic Scientific Research of Central Colleges, China University of Geosciences (Wuhan) (Nos. CUG090103 and G1323521132), and the 111 Project (No. B08030). Professor Robert K. Booth and Professor Edward A.D. Mitchell helped with species identifications, Professor Booth also helped with initial statistical analysis and constructive comments on the research. We thank Jiantao Xue, Ze Zhang, Huan Yang and Zhiqi Zhang for their assistance of field works. Qianfan Zhang and Rong Zhu helped dealing with the processing of samples in the laboratory.Author contributions: YQ designed and led the study, conducted all laboratory and microscopic analysis and wrote the paper. RJP conducted all statistical analysis and wrote the paper with YQ. YG, XH and HW helped with the field work.

Author contributions: YQ designed and led the study, conducted all laboratory and microscopic analysis and wrote the paper. RJP conducted all statistical analysis and wrote the paper with YQ. YG, XH and HW helped with the field work.

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