Rapid detection of bacteria in drinking water and water contamination case studies

Abstract

Water systems are inherently vulnerable to physical, chemical and biologic threats that might compromise a systems’ ability to reliably deliver safe water. The ability of a water supply to provide water to its customers can be compromised by destroying or disrupting key physical elements of the water system. However, contamination is generally viewed as the most serious potential terrorist threat to water systems. Chemical or biologic agents could spread throughout a distribution system and result in sickness or death among the consumers and for some agents the presence of the contaminant might not be known until emergency rooms report an increase in patients with a particular set of symptoms. Even without serious health impacts, just the knowledge that a water system had been breached could seriously undermine consumer confidence in public water supplies. Therefore, the ability to rapidly detect contamination, especially microbiological contamination, is highly desirable. The authors summarize water contamination case studies and discuss a technique for identifying microbiological contamination based on ATP bioluminescence. This assay allows an estimation of bacterial populations within minutes and can be applied using a local platform. Previous ATP-based methods requires one hour, one liter of water, and has a sensitivity of 100000 cells for detection. The improved method discussed here is 100 times more sensitive, requires one-hundredth of the sample volume, and is over 10 times faster than standard method. T\his technique has a great deal of potential for application in situations in which a water system has been compromised.

Keywords

drinking water / bacteria / waste water treatment plants

References

[1] Abbaszadegan M, Alum A (2004). Microbiological contaminants and threats of concern. Mays L W, ed. Water Supply Systems Security. New York: McGraw-Hill, 2.1–2.12

[2] Angulo F J, Tippen S, Sharp D J, Payne B J, Collier C, Hill J E, Barrett T J, Clark R M, Geldreich E E, Donnell H D Jr, Swerdlow D L (1997). A community waterborne outbreak of salmonellosis and the effectiveness of a boil water order. Am J Public Health, 87(4): 580–584

[3] APHA (American Public Health Association), American Water Works Association (AWWA), Water Environment Federation (WEF) (1995). Standard Methods for the Examination of Water and Wastewater. Franson M A, 19th ed. Washington DC: American Public Health Association

[4] ASTM D 4012.81 (2003). Standard Test Method for Adenosine Triphosphate (ATP) Contents of Microorganisms in Water

[5] Burrows W D, Renner S E (1998). Biological Warfare Agents as Potable Water Threats. US Army Combined Arms Support Command, Fort Lee, USA, 10

[6] Burrows W D, Renner S E (1999). Biological warfare agents as threats to potable water. Environ Health Perspect, 107(12): 975–984

[7] Bushon R N, Brady A M, Likirdopulos C A, Cireddu J V (2009). Rapid detection of Escherichia coli and Enterococci in recreational water using an immunomagnetic separation/adenosine triphosphate technique. J Appl Microbiol, 106(2): 432–441

[8] Clark R M (2002). Assessing the Etiology of a Waterborne Outbreak: Public Health Emergency or Covert Attack. In: Hatchett J, ed. Proceedings of the First Water Security Summitt, Haested, Heasted Methods, Waterbury, CT, 170–179

[9] Clark R M (2011). US water and wastewater critical infrastructure. Clark R M, Hakim S, Ostfeld A, eds. Handbook for Securing Water and Wastewater Systems. Heidelberg: Springer-Science

[10] Clark R M, Deininger R A (2000). Protecting the nation’s critical infrastructure: The vulnerability of US water supply systems. J Contingencies Crisis Manage, 8(2): 73–80

[11] Clark R M, Deininger R A (2001). Minimizing the Vulnerability of Water Supplies to Natural and Terrorist Threats. In: Proceedings of the American Water Works Association’s IMTech Conference, Atlanta, USA, <month>April</month><day>8–11</day> 2001, 1–20

[12] Clark R M, Geldreich E E, Fox K R, Rice E W, Johnson C H, Goodrich J A, Bsarnick J A, Abdesaken F (1996). Tracking a Salmonella serovar Typhimurium outbreak in Gideon, Missouri: Role of contaminant propagation modeling. Journal of Water Supply: Research and Technology, 45(4): 171–183

[13] Clark R M, Rossman L, Wymer L (1995). Modeling distribution system water quality: Regulatory implications. J Water Resour Plan Manage, 121(6): 423–428

[14] Deininger R A, Lee J (1998). Rapid Determination of Bacteria in Water. In: Proceedings Water Quality Technology Conference, American Water Works Association, November 1–4, San Diego, USA

[15] Deininger R A, Lee J (2007). Rapid Determination of Bacterial Loads for the Assessment of Water Quality. Detection Technologies, November 2, San Diego, USA

[16] Deininger R A, Lee J, Klangsin P (1997). Rapid onsite determination of bacteria in a water distribution system. American Water Works Association, June 7–8, Norfolk, USA

[17] Delahaye E, Welté B, Levi Y, Leblon G, Montiel A (2003). An ATP-based method for monitoring the microbiological drinking water quality in a distribution network. Water Res, 37(15): 3689–3696

[18] Fennel H, James D B, Morris J (1974). Pollution of a storage reservoir by roosting gulls. Journal of Society of Water Treatment Exam, 23: 5–24

[19] Field M S (2004). Assessing the risks to drinking-water supplies from terrorists attacks. Mays L W, ed. Water Supply Systems Security, New York: McGraw-Hill, 6.1–6.26

[20] Fox K R, Lytle D A (1996). Milwaukee’s crypto outbreak investigations and recommendations. J Am Water Works Assoc, 88(9): 87–94

[21] Frundzhyan V, Ugarova N (2007). Bioluminescent assay of total bacterial contamination of drinking water. Luminescence, 22(3): 241–244

[22] Geldreich E E, Fox K R, Goodrich J A, Rice E W, Clark R M, Swerdlow D L (1992). Searching for a water supply connection in the cabool, missouri disease outbreak of Escherichia coli O 157:H7. Water Res, 26 (8): 1127–1137

[23] Gleick P H (2006). Water and terrorism. Water Policy, 8(6): 481–503

[24] Grayman W M, Clark R M, Harding B L, Maslia M L, Aramini J (2004). Reconstructing historical contamination events. Mays L, ed. Water Supply Systems Security. New York: McGraw-Hill, 10.1–10.55

[25] Hrudey S E, Hrudey E J (2004). Safe Drinking Water: Lessons from Recent Outbreaks in Affluent Nations. London: IWA Publishing

[26] Lee C, Griffith J, Kaiser W, Jay J (2010). Cov-IMS/ATP enables rapid in-field detection and quantification of Escherichia coli and Enterococcus spp. in freshwater and marine environment. Journal of Applied Microbiology

[27] Lee J, Deininger R A (1999). A rapid method for detecting bacteria in drinking water. Journal of Rapid Method and Automation in Microbiology, 7: 135–145

[28] Lee J, Deininger R A (2001). Rapid quantification of viable bacteria in water using an ATP assay. New Products, <month>October</month>2001

[29] Lee J, Deininger R A (2010). Real time determination of the efficacy of residual disinfection to limit sewage contamination in a water distribution system using filtration based luminescence. Water Environ Res, 5(82): 474–478

[30] Meier T R, Maute C J, Cadillac J M, Lee J Y, Righter D J, Hugunin K M, Deininger R A, Dysko R C (2008). Quantification, distribution and possible source of bacterial bio-film in rodent automated watering systems. J Am Assoc Lab Anim Sci, 2(47): 63–70

[31] Michigan Department of Community Health (2002). New Horizons Diagnostics, Profile–1, Bioluminometer, 9110 Red Branch Road, Columbia, 21045, USA

[32] Register F (1990). National primary drinking water regulations: Analytical techniques. Coliform Bacteria, 55: 22752–22756

[33] Rice E W, Clark R M, Johnson C H (1999). Chlorine inactivation of Escherichia coli O 157:H7. Emerg Infect Dis, 5 (3): 461–463

[34] Rossman L A, Clark R M, Grayman W M (1994). Modeling chlorine residuals in drinking water distribution systems. J Environ Eng, 120(4): 803–820

[35] Skala M F (1994). Waterborne Salmonella outbreak in southeastern Missouri. Missouri Epidemiologist, 17(2): 1–2

[36] The Walkerton Herald Times (2000). Wednesday August 2nd, 31(141)

[37] Times New York (1986). White House water cut off temporarily. July 10th, 16

[38] Wall Street Journal (2008). China Turns Away Shipment of Evian. Australian Seafood, May 30th, 2007

[39] Webster A H, Lee J, Deininger R A (2005). Rapid assessment of microbial hazards in metal working fluids. J Occup Environ Hyg, 2(4): 213–218

[40] Weil P W J J, van der Kooij D (2010). Adenosine triphosphate (ATP) as a parameter to determine microbiological activity in distributed drinking water. Water Research, 44(17): 4860–4867

[41] Williams P, Wallace D (1989). Unit 731, the Japanese Army’s Secret of Secrets. London: Hodder & Stoughton

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