Frontiers of Agriculture in China >

2007 , Vol. 1 >Issue 3: 352 - 356

DOI: https://doi.org/10.1007/s11703-007-0059-y

mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP

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  • 1.Department of Animal Science & Technology, Agricultural University of Hebei, Baoding 071001, China; 2.Department of Animal Science Hebei Normal University of Science & Technology, Changli 066600, China

Published date: 05 Sep 2007

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Abstract

The polymorphism of mitochondrial DNA (mtDNA) D-loop of 83 individuals from nine Chinese indigenous sheep breeds and two imported sheep breeds was studied with five endonucleases, Hinf I, Msp I, Sau3A I, Xsp I and Taq I, using PCR-RFLP. The results indicated that there existed two basic haplotypes in the region of mtDNA D-loop. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors. The average polymorphic degree (π value = 0.0421%) of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very low.

Cite this article

LI Xianglong, ZHANG Zengli, JIA Qing, WANG Lize, GONG Yuanfang, LIU Zhengzhu . mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP[J]. Frontiers of Agriculture in China, 2007 , 1(3) : 352 -356 . DOI: 10.1007/s11703-007-0059-y

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