mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP

LI Xianglong; ZHANG Zengli; JIA Qing; WANG Lize; GONG Yuanfang; LIU Zhengzhu

doi:10.1007/s11703-007-0059-y

Front. Agric. China ›› 2007, Vol. 1 ›› Issue (3) :352 -356. DOI: 10.1007/s11703-007-0059-y

mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP

LI Xianglong¹, ZHANG Zengli¹, JIA Qing¹, WANG Lize¹, GONG Yuanfang², LIU Zhengzhu²

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Abstract

The polymorphism of mitochondrial DNA (mtDNA) D-loop of 83 individuals from nine Chinese indigenous sheep breeds and two imported sheep breeds was studied with five endonucleases, Hinf I, Msp I, Sau3A I, Xsp I and Taq I, using PCR-RFLP. The results indicated that there existed two basic haplotypes in the region of mtDNA D-loop. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors. The average polymorphic degree (π value = 0.0421%) of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very low.

Keywords

indigenous sheep breeds, mtDNA, D-loop, PCR-RFLP

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LI Xianglong, ZHANG Zengli, JIA Qing, WANG Lize, GONG Yuanfang, LIU Zhengzhu. mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP. Front. Agric. China, 2007, 1(3): 352-356 DOI:10.1007/s11703-007-0059-y