Frontiers of Agriculture in China >

2011 , Vol. 5 >Issue 4: 576 - 580

DOI: https://doi.org/10.1007/s11703-011-1119-x

RESEARCH ARTICLE

Construction of a Fosmid genomic library of Streptomyces roseoflavus Men-myco-93-63

  • Weiming SUN 1,2 ,
  • Wei GUO , 1,2,3 ,
  • Daqun LIU , 1 ,
  • Tinghui LIU 1 ,
  • Lina FENG 1 ,
  • Lianna LIU 2 ,
  • Yaning LI 1 ,
  • Yakun ZHANG 2
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  • 1. Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, College of Plant Protection, Agricultural University of Hebei, Baoding 071000, China
  • 2. College of Life Sciences, Agricultural University of Hebei, Baoding 071000, China
  • 3. National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071000, China

Received date: 29 Mar 2011

Accepted date: 12 Apr 2011

Published date: 05 Dec 2011

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
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Abstract

To clone the antibiotic biosynthesis gene cluster of Streptomyces roseoflavus Men-myco-93-63, we constructed a Fosmid genomic library. The genomic DNA of the strain Men-myco-93-63 was isolated by the modified CTAB procedure, and the size of most genomic DNA fragments was larger than 150 kb. Then, a Fosmid genomic library containing more than 6000 clones was constructed. The average size of the inserted DNA in recombinant plasmids was 38.1 kb, and the probability of harboring any gene in the genome of the strain Men-myco-93-63 was 99.99%. The library coverage was at least a 10-fold genome equivalent. Therefore, the constructed Fosmid library meets the requirements as a standard genomic library

Key words： Streptomyces roseoflavus; Fosmid; genomic library

Cite this article

Weiming SUN , Wei GUO , Daqun LIU , Tinghui LIU , Lina FENG , Lianna LIU , Yaning LI , Yakun ZHANG . Construction of a Fosmid genomic library of Streptomyces roseoflavus Men-myco-93-63[J]. Frontiers of Agriculture in China, 2011 , 5(4) : 576 -580 . DOI: 10.1007/s11703-011-1119-x

Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant No. 30800734) and the National Natural Science Foundation of Hebei Province, China (Grant No. C2011204114).

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