Construction of a Fosmid genomic library of Streptomyces roseoflavus Men-myco-93-63

Abstract

To clone the antibiotic biosynthesis gene cluster of Streptomyces roseoflavus Men-myco-93-63, we constructed a Fosmid genomic library. The genomic DNA of the strain Men-myco-93-63 was isolated by the modified CTAB procedure, and the size of most genomic DNA fragments was larger than 150 kb. Then, a Fosmid genomic library containing more than 6000 clones was constructed. The average size of the inserted DNA in recombinant plasmids was 38.1 kb, and the probability of harboring any gene in the genome of the strain Men-myco-93-63 was 99.99%. The library coverage was at least a 10-fold genome equivalent. Therefore, the constructed Fosmid library meets the requirements as a standard genomic library

Keywords

Streptomyces roseoflavus / Fosmid / genomic library

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant No. 30800734) and the National Natural Science Foundation of Hebei Province, China (Grant No. C2011204114).

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