Research article

Cellular immune responses of BALB/c mice induced by intramuscular injection of PRRSV-ORF5 DNA vaccine with different doses

  • Anchun CHENG 1,2 ,
  • Mingshu WANG 1,2 ,
  • Xiwen CHEN , 1,2,3 ,
  • Nigen XINI 2 ,
  • Wenbo DOU 2 ,
  • Xuemei LI 2 ,
  • Wumei LIU 2 ,
  • Gang WANG 2 ,
  • Pingying ZHANG 2
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  • 1 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya’an 625014, China
  • 2College of Animal Science and Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China
  • 3Institute of Applied Animal Technology, Mianyang Normal College, Mianyang 621000, China, Key Laboratory for Molecular Biology and Biopharmaceuticals, Mianyang Normal College, Mianyang, Sichuan 621000, China , The first three authors contribute equally to this work.

Copyright

2007 Higher Education Press

Abstract

BALB/c mice were immunized with 50 µg, 100 µg, 200 µg of pcDNA-PRRSV-ORF5 DNA vaccine respectively by intramuscular injection, with PBS and pcDNA3.1(+) as controls. Fluorescence activated cell Sorter (FACS) was used to detect the number of CD+4 and CD+8 T-lymphocytes. T-lymphocyte proliferation test was used to detect proliferation of the T-lymphocyte cells in peripheral blood lymphocytes of mice vaccinated with pcDNA-PRRSV-ORF5 DNA vaccine. The results showed that the difference in ConA response to T-lymphocytes in blood was highly significant between all experimental groups and the control group (P<0.01). The number of CD4+ T-lymphocytes in experimental groups was significantly higher than that of the control group 7 d after vaccination. The number of CD8+ T-lymphocytes in the experimental groups was higher than that of the control group 28 d after vaccination. Mice immunized with a higher dose (200 µg) of DNA vaccine demonstrated higher cellular immune response than those immunized with a lower dose (100 µg, 50 µg) of DNA vaccine. The results demonstrated that pcDNA-PRRSV-ORF5 DNA vaccine could induce a good cellular immune response which may be dose-dependent.

Cite this article

Anchun CHENG , Mingshu WANG , Xiwen CHEN , Nigen XINI , Wenbo DOU , Xuemei LI , Wumei LIU , Gang WANG , Pingying ZHANG . Cellular immune responses of BALB/c mice induced by intramuscular injection of PRRSV-ORF5 DNA vaccine with different doses[J]. Frontiers of Agriculture in China, 2007 , 1(1) : 105 -110 . DOI: 10.1007/s11703-007-0020-0

Acknowledgements

This work was supported by Sichuan Bio-tech Project (No. 01NG018-03), the National State Key Program Project of Science and Technology (No. 2004BA901A03), Program for New Century Excellent Talents in University, Ministry of Education, China (No. NCET-04-0906).
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