Cellular immune responses of BALB/c mice induced by intramuscular injection of PRRSV-ORF5 DNA vaccine with different doses

Anchun CHENG, Mingshu WANG, Xiwen CHEN, Nigen XINI, Wenbo DOU, Xuemei LI, Wumei LIU, Gang WANG, Pingying ZHANG

Front. Agric. China ›› 2007, Vol. 1 ›› Issue (1) : 105-110.

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PDF(350 KB)
Front. Agric. China ›› 2007, Vol. 1 ›› Issue (1) : 105-110. DOI: 10.1007/s11703-007-0020-0
Research article
Research article

Cellular immune responses of BALB/c mice induced by intramuscular injection of PRRSV-ORF5 DNA vaccine with different doses

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Abstract

BALB/c mice were immunized with 50 µg, 100 µg, 200 µg of pcDNA-PRRSV-ORF5 DNA vaccine respectively by intramuscular injection, with PBS and pcDNA3.1(+) as controls. Fluorescence activated cell Sorter (FACS) was used to detect the number of CD+4 and CD+8 T-lymphocytes. T-lymphocyte proliferation test was used to detect proliferation of the T-lymphocyte cells in peripheral blood lymphocytes of mice vaccinated with pcDNA-PRRSV-ORF5 DNA vaccine. The results showed that the difference in ConA response to T-lymphocytes in blood was highly significant between all experimental groups and the control group (P<0.01). The number of CD4+ T-lymphocytes in experimental groups was significantly higher than that of the control group 7 d after vaccination. The number of CD8+ T-lymphocytes in the experimental groups was higher than that of the control group 28 d after vaccination. Mice immunized with a higher dose (200 µg) of DNA vaccine demonstrated higher cellular immune response than those immunized with a lower dose (100 µg, 50 µg) of DNA vaccine. The results demonstrated that pcDNA-PRRSV-ORF5 DNA vaccine could induce a good cellular immune response which may be dose-dependent.

Keywords

PRRSV / ORF5 DNA vaccine / cellular immune response / mice

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Anchun CHENG, Mingshu WANG, Xiwen CHEN, Nigen XINI, Wenbo DOU, Xuemei LI, Wumei LIU, Gang WANG, Pingying ZHANG. Cellular immune responses of BALB/c mice induced by intramuscular injection of PRRSV-ORF5 DNA vaccine with different doses. Front. Agric. China, 2007, 1(1): 105‒110 https://doi.org/10.1007/s11703-007-0020-0

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Acknowledgements

This work was supported by Sichuan Bio-tech Project (No. 01NG018-03), the National State Key Program Project of Science and Technology (No. 2004BA901A03), Program for New Century Excellent Talents in University, Ministry of Education, China (No. NCET-04-0906).

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