MPN-PCR enumeration of Campylobacter spp. in raw chicken meats and by-products

Abstract

The goal of this study is to enumerate Campylobacter in chicken meats and by-products. In the current investigation, results showed that raw chicken meats and chicken by-products were contaminated with Campylobacter ranging from<3 to 4600 MPN·g^-1. Campylobacter jejuni showed a higher number compared to Campylobacter coli in the chicken samples. The current study showed that the percentage of chicken livers and gizzards harbored a higher number of Campylobacter (10³–10⁴ MPN·g^-1) than other chicken parts at 33.3% and 9.2%, respectively. The different concentrations of Campylobacter between chicken meats and chicken by-products reflect the differences in the contamination level. The data on Campylobacter concentration in chicken meats and by-products will be useful in risk analysis.

Key words： MPN-PCR (most-probable-number PCR); Campylobacter; chicken meats; chicken by-products

Acknowledgements

This study was supported by the Science Fund (No. 05-01-04-SF0379) from the Ministry of Science, Technology and Innovation, Malaysia and in-part by a Grant-in-Aid for Scientific Research (No. KAKENHI 191010) from the Japan Society for the Promotion of Sciences. We wish to thank the Faculty of Veterinary, UPM for generously supplying the fresh horse blood.

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