RESEARCH ARTICLE

MPN-PCR enumeration of Campylobacter spp. in raw chicken meats and by-products

  • John Yew Huat TANG 1 ,
  • Farinazleen Mohamad GHAZALI 2 ,
  • Abdul Aziz SALEHA, 3 ,
  • Yoshitsugu NAKAGUCHI 4 ,
  • Mitsuaki NISHIBUCHI 4 ,
  • Son RADU , 2
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  • 1. Faculty of Food Technology, Universiti Sultan Zainal Abidin, 20400 Kuala Terengganu, Terengganu, Malaysia
  • 2. Centre of Excellence for Food Safety Research, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • 3. Department of Pathology-Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • 4. Center for Southeast Asian Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto, Japan

Received date: 10 Apr 2010

Accepted date: 26 Apr 2010

Published date: 05 Dec 2010

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

The goal of this study is to enumerate Campylobacter in chicken meats and by-products. In the current investigation, results showed that raw chicken meats and chicken by-products were contaminated with Campylobacter ranging from<3 to 4600 MPN·g-1. Campylobacter jejuni showed a higher number compared to Campylobacter coli in the chicken samples. The current study showed that the percentage of chicken livers and gizzards harbored a higher number of Campylobacter (103–104 MPN·g-1) than other chicken parts at 33.3% and 9.2%, respectively. The different concentrations of Campylobacter between chicken meats and chicken by-products reflect the differences in the contamination level. The data on Campylobacter concentration in chicken meats and by-products will be useful in risk analysis.

Cite this article

John Yew Huat TANG , Farinazleen Mohamad GHAZALI , Abdul Aziz SALEHA, , Yoshitsugu NAKAGUCHI , Mitsuaki NISHIBUCHI , Son RADU . MPN-PCR enumeration of Campylobacter spp. in raw chicken meats and by-products[J]. Frontiers of Agriculture in China, 2010 , 4(4) : 501 -506 . DOI: 10.1007/s11703-010-1042-6

Acknowledgements

This study was supported by the Science Fund (No. 05-01-04-SF0379) from the Ministry of Science, Technology and Innovation, Malaysia and in-part by a Grant-in-Aid for Scientific Research (No. KAKENHI 191010) from the Japan Society for the Promotion of Sciences. We wish to thank the Faculty of Veterinary, UPM for generously supplying the fresh horse blood.
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