MPN-PCR enumeration of Campylobacter spp. in raw chicken meats and by-products

Abstract

The goal of this study is to enumerate Campylobacter in chicken meats and by-products. In the current investigation, results showed that raw chicken meats and chicken by-products were contaminated with Campylobacter ranging from<3 to 4600 MPN·g^-1. Campylobacter jejuni showed a higher number compared to Campylobacter coli in the chicken samples. The current study showed that the percentage of chicken livers and gizzards harbored a higher number of Campylobacter (10³–10⁴ MPN·g^-1) than other chicken parts at 33.3% and 9.2%, respectively. The different concentrations of Campylobacter between chicken meats and chicken by-products reflect the differences in the contamination level. The data on Campylobacter concentration in chicken meats and by-products will be useful in risk analysis.

Keywords

MPN-PCR (most-probable-number PCR) / Campylobacter / chicken meats / chicken by-products

References

[1] Arsenault J, Letellier A, Quessy S, Boulianne M (2007). Prevalence and risk factors for Salmonella and Campylobacter spp. carcass contamination in broiler chickens slaughtered in Quebec, Canada. J Food Prot, 70(8): 1820–1828

[2] Berndtson E, Tivemo M, Engvall A (1992). Distribution and numbers of Campylobacter in newly slaughtered broiler chickens and hens. Int J Food Microbiol, 15(1–2): 45–50

[3] Black R E, Levine M M, Clements M L, Hughes T P, Blaser M J (1988). Experimental Campylobacter jejuni infection in humans. J Infect Dis, 157(3): 472–479

[4] Campbell D F, Johnston R W, Campbell G S, McClain D, Macaluso J F (1983). The microbiology of raw, eviscerated chickens: a ten year comparison. Poult Sci, 81: 414–421

[5] Chai L C, Robin T, Ragavan U M, Gunsalam J W, Bakar F A, Ghazali F M, Radu S, Kumar M P (2007). Thermophilic Campylobacter spp. in salad vegetables in Malaysia. Int J Food Microbiol, 117(1): 106–111

[6] Denis M, Refrégier-Petton J, Laisney M J, Ermel G, Salvat G (2001). Campylobacter contamination in French chicken production from farm to consumers. Use of a PCR assay for detection and identification of Campylobacter jejuni and Camp. coli. J Appl Microbiol, 91(2): 255–267

[7] Fischer A R, De Jong A E I, Van Asselt E D, De Jonge R, Frewer L J, Nauta M J (2007). Food safety in the domestic environment: an interdisciplinary investigation of microbial hazards during food preparation. Risk Anal, 27(4): 1065–1082

[8] Gonzalez I, Grant K A, Richardson P T, Park S F, Collins M D (1997). Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant. J Clin Microbiol, 35(3): 759–763

[9] Han K, Jang S S, Choo E, Heu S, Ryu S (2007). Prevalence, genetic diversity, and antibiotic resistance patterns of Campylobacter jejuni from retail raw chickens in Korea. Int J Food Microbiol, 114(1): 50–59

[10] Harrison W A, Griffith C J, Tennant D, Peters A C (2001). Incidence of Campylobacter and Salmonella isolated from retail chicken and associated packaging in South Wales. Lett Appl Microbiol, 33(6): 450–454

[11] Havelaar A H, Mangen M J J, De Koeijer A A, Bogaardt M J, Evers E G, Jacobs-Reitsma W F, Van Pelt W, Wagenaar J A, De Wit G A, Van der Zee H, Nauta M J (2007). Effectiveness and efficiency of controlling Campylobacter on broiler chicken meat. Risk Anal, 27(4): 831–844

[12] Klein G, Reich F, Beckmann L, Atanassova V (2007). Quantification of thermophilic Campylobacter spp. in broilers during meat processing. Antonie van Leeuwenhoek, 92(3): 267–273

[13] Lindblad M, Lindmark H, Lambertz S T, Lindqvist R (2006). Microbiological baseline study of broiler chickens at Swedish slaughterhouses. J Food Prot, 69(12): 2875–2882

[14] Linton D, Lawson A J, Owen R J, Stanley J (1997). PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples. J Clin Microbiol, 35(10): 2568–2572

[15] Linton D, Owen R J, Stanley J (1996). Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals. Res Microbiol, 147(9): 707–718

[16] Luber P, Bartelt E (2007). Enumeration of Campylobacter spp. on the surface and within chicken breast fillets. J Appl Microbiol, 102(2): 313–318

[17] Manfreda G, De Cesare A, Bondioli V, Stern N J, Franchini A (2006). Enumeration and identity of Campylobacter spp. in Italian broilers. Poult Sci, 85(3): 556–562

[18] Meldrum R J, Wilson I G (2007). Salmonella and Campylobacter in United Kingdom retail raw chicken in 2005. J Food Prot, 70(8): 1937–1939

[19] Nauta M, van der Fels-Klerx I, Havelaar A (2005). A poultry-processing model for quantitative microbiological risk assessment. Risk Anal, 25(1): 85–98

[20] Ono K, Yamamoto K (1999). Contamination of meat with Campylobacter jejuni in Saitama, Japan. Int J Food Microbiol, 47(3): 211–219

[21] Park R W A, Griffiths P L, Moreno G S (1991). Sources and survival of Campylobacter: relevance to enteritis and the food industry. Society for Applied Bacteriology Symposium series, 70: 97S–106S

[22] Phillips C A (1995). Incidence, epidemiology and prevention of foodborne campylobacter species. Trends Food Sci Technol, 6(3): 83–87

[23] Pointon A, Sexton M, Dowsett P, Saputra T, Kiermeier A, Lorimer M, Holds G, Arnold G, Davos D, Combs B, Fabiansson S, Raven G, McKenzie H, Chapman A, Sumner J (2008). A baseline survey of the microbiological quality of chicken portions and carcasses at retail in two Australian states (2005 to 2006). J Food Prot, 71(6): 1123–1134

[24] Rodrigo S, Adesiyun A, Asgarali Z, Swanston W (2005). Prevalence of Campylobacter spp. on chickens from selected retail processors in Trinidad. Food Microbiol, 22(1): 125–131

[25] Rosenquist H, Nielsen N L, Sommer H M, Nørrung B, Christensen B B (2003). Quantitative risk assessment of human campylobacteriosis associated with thermophilic Campylobacter species in chickens. Int J Food Microbiol, 83(1): 87–103

[26] Rosenquist H, Sommer H M, Nielsen N L, Christensen B B (2006). The effect of slaughter operations on the contamination of chicken carcasses with thermotolerant Campylobacter. Int J Food Microbiol, 108(2): 226–232

[27] Saleha A A (2002). Isolation and characterization of Campylobacter jejuni from broiler chickens in Malaysia. Int J Poult Sci, 1(4): 94–97

[28] Saleha A A (2004). Epidemiological study on the colonization of chickens with Campylobacter in broiler farms in Malaysia: Possible risk and management factors. Int J Poult Sci, 3(2): 129–134

[29] Sallam K I (2007). Prevalence of Campylobacter in chicken and chicken by-products retailed in Sapporo area, Hokkaido, Japan. Food Control, 18(9): 1113–1120

[30] Solomon E B, Hoover D G (1999). Campylobacter jejuni: a paradox. J Food Saf ety, 19(2): 121–136

[31] Son R, Karim M I A, Rusul G, Yusoff K (1996). Plasmids and antimicrobial resistance among Campylobacter jejuni isolated from retail fresh poultry. Asia Pac J Mol Biol Biotechnol, 4: 106–111

[32] Stern N J, Line J E, Chen H C(2001),Campylobacter. In: Downes F P, Ito K, eds. Compendium of Methods for the Microbiological Examination of Foods. 4th ed. Washington D C: American Public Health Association, 301–310

[33] Tang J Y H, Mohamad Ghazali F, Saleha A A, Nishibuchi M, Son R (2009). Comparison of thermophilic Campylobacter spp. occurrence in two types of retail chicken samples. Int Food Res J, 16: 277–288

[34] Tang J Y H, Saleha A A, Jalila A, Mohamad Ghazali F, Tuan Zainazor T C, Noorlis A, Sandra A, Nishibuchi M, Radu S (2010). Thermophilic Campylobacter spp. occurrence on chickens at farm, slaughter house and retail. Int J Poult Sci, 9: 134–138

[35] Tauxe R V (1992). Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations. In: Nachamkin I, Blaser M J, Tompkins L, eds. Campylobacter jejuni: Current Status and Future Trends. Washington D C: American Society for Microbiology Press, 9–19

[36] Uyttendaele M, Baert K, Ghafir Y, Daube G, De Zutter L, Herman L, Dierick K, Pierard D, Dubois J J, Horion B, Debevere J (2006). Quantitative risk assessment of Campylobacter spp. in poultry based meat preparations as one of the factors to support the development of risk-based microbiological criteria in Belgium. Int J Food Microbiol, 111(2): 149–163

[37] Waldroup A L, Rathgeber B M, Forsythe R H (1992). Effects of six modifications on the incidence and levels of spoilage and pathogenic organisms on commercial processed postchill broilers. J Appl Poult Res, 2: 111–116

[38] Whyte P, McGill K, Cowley D, Madden R H, Moran L, Scates P, Carroll C, O’Leary A, Fanning S, Collins J D, McNamara E, Moore J E, Cormican M (2004). Occurrence of Campylobacter in retail foods in Ireland. Int J Food Microbiol, 95(2): 111–118

Acknowledgements

This study was supported by the Science Fund (No. 05-01-04-SF0379) from the Ministry of Science, Technology and Innovation, Malaysia and in-part by a Grant-in-Aid for Scientific Research (No. KAKENHI 191010) from the Japan Society for the Promotion of Sciences. We wish to thank the Faculty of Veterinary, UPM for generously supplying the fresh horse blood.

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