Initial function determination for the open reading frame (ORF) region of Pib gene via rice transformation

Abstract

Three plant binary expression vectors—pNAR501, pNAR502 and pNAR503—were constructed, carrying fragments of exon2-exon3, 5 2partial deletion exon1 and 5 2partial deletion exon1-exon2-exon3 of Pib gene driven by 35S. These three vectors were transformed into the japonica rice variety Nipponbare through agrobacterium-mediated transformation. More than 30 transgenic rice plants were obtained and confirmed by polymerase chain reaction (PCR), Southern hybridization and the hygromycin resistance test in seed germination of their progeny. A rice blast resistance test for in vitro leaves of T_o transgenic plants in the tillering stage showed higher resistance to the races of E1, F1 and G1 of rice blast than that of the control Nipponbare. However, results of rice blast resistance test for seedlings of T_l transgenic plants in the 3- to 4-leaf stage were different. All T_l transgenic seedlings had a lower level of resistance to E1, F1and G1 races than that of the control Nipponbare.

Keywords

rice, rice blast, Pib gene