Characterization of eosinophil populations in a murine model of type 2 airway inflammation via flow cytometry

Abstract

Purpose: This study aims to optimize experimental conditions to obtain a larger quantity of eosinophils from the type 2 inflammation mouse model, which will serve as a basis for further investigation into the role and mechanisms of eosinophils in type 2 inflammation.

Methods: We used a mouse model of type 2 inflammation and isolated potential eosinophil-resident tissues (bone marrow, spleen, and nasal mucosa) for flow cytometry analysis. By combining forward scatter, side scatter, and the cell population identified as CD45⁺ (protein tyrosine phosphatase receptor type C) CD11b⁺ (integrin alpha-M) Ly6G⁻ (lymphocyte antigen 6 family member G) Siglec-F⁺ (sialic acid-binding immunoglobulin-like lectin F), we characterized eosinophils.

Results: The results showed that the number and proportion of eosinophils in bone marrow were numerically higher compared to the spleen and nasal mucosa. Further exploration of conditions may provide guarantees for the later development of mechanism research.

Conclusion: These findings provide clues for further optimizing experimental conditions to obtain sufficient eosinophils from bone marrow for mechanistic studies. They also offer a potential framework for exploring phenotypic heterogeneity and functional studies of eosinophils in various tissues.

Keywords

eosinophils / murine model / type 2 inflammation

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The Author(s). Eye & ENT Research published by John Wiley & Sons Australia, Ltd on behalf of Higher Education Press.