The changes of the levels of LTC4, PGI2 and TXA2 in the liver tissue in SD rats with GalN/LPS-induced acute liver injury was studied with radioimmunoassay (RIA). As a result, 12 h after the administration of GaIN/LPS, serum AST (398±37 u), ALT (565 ±43 u) increased (P<0.001) and the concentration of TXA. (12188±588 pg/g · w · wt) in liver tissue increased significantly (P<0.001), while the content of LTC4 (9713±3557 ng/g · w · wt) and PGI2(1748±560 pg/g · w · wt) in liver tissue were not obviously changed (P>0.05) and the inflammatory changes of the pathological findings were observed. The improvement of serum ALT (330±168 u) (P<0.05) and AST (273±124 u) (P<0.05) and histopathological damage was observed after the administration of di-ethylcarbamazine (DEC), a LTA4 synthesis inhibitor, the liver TXA2 (12740±699) concentration significantly increased (P<0.001), while the levels of LTC4 (8179±1653) and PGI2 (2320±630) were not obviously changed. Serum ALT (536±74 u) and AST (416±41 u) (P>0.05) levels and histopathology did not change with administration of indomethacin, a cyclooxygenase inhibitor, but the liver LTC4 (12166±1327) contents increased (P<0. 05) and TXA2 (1868±791) reduced significantly (P<0.001). The present study suggests that arachidonic acid metabolism in rats with acute liver injury are significantly abnormal. Leukotrienes and thromboxane are important inflammatory mediators in the liver injury.