Single-cell and spatial alterations of neural cells and circuits in clinical and translational medicine

William Wang, Xuanqi Liu, Diane Catherine Wang

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Clinical and Translational Discovery ›› 2024, Vol. 4 ›› Issue (3) : e298. DOI: 10.1002/ctd2.298
EDITORIAL

Single-cell and spatial alterations of neural cells and circuits in clinical and translational medicine

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Abstract

The spatiotemporal heterogeneity of neurons, circuits and regulators is being uncovered at a single-cell level, from the single-cell gene expression to functional regulations. The classifications, architectonics and functional communications amongst neural cells and circuits within the brain can be clearly delineated using single-cell multiomics and transomics. This Editorial highlights the spatiotemporal heterogeneity of neurons and circuits as well as regulators, initiates the translation of neuronal diversity and spatial organisation at single-cell levels into clinical considerations, and enables the discovery and development of new therapies for neurological diseases. It is predicted that single-cell and spatial multiomics will be integrated with metabolomic profiles and corresponding gene epigenetic modifications. The interactions amongst DNAs, RNAs and proteins in a cell provide details of intracellular functional regulations and new opportunities for the translation of temporospatial diversity of neural cell subtypes/states into clinical practice. The application of single-cell multiomics with four-dimensional genome to the human pathological brain will lead us to a new milestone of the diagnosis and treatment.

Keywords

clinical / disease / medicine / single-cell / translational

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William Wang, Xuanqi Liu, Diane Catherine Wang. Single-cell and spatial alterations of neural cells and circuits in clinical and translational medicine. Clinical and Translational Discovery, 2024, 4(3): e298 https://doi.org/10.1002/ctd2.298

References

[1]
Zhang L, Chen D, Song D, et al. Clinical and translational values of spatial transcriptomics. Signal Transduct Target Ther. 2022;7(1):111.
CrossRef Google scholar
[2]
Liu X, Jiang Y, Song D, et al. Clinical challenges of tissue preparation for spatial transcriptome. Clin Transl Med. 2022;12(1):e669.
CrossRef Google scholar
[3]
BRAIN Initiative Cell Census Network (BICCN). A multimodal cell census and atlas of the mammalian primary motor cortex. Nature. 2021;598(7879):86-102.
CrossRef Google scholar
[4]
Liu H, Zhou J, Tian W, et al. DNA methylation atlas of the mouse brain at single-cell resolution. Nature. 2021;598(7879):120-128.
CrossRef Google scholar
[5]
Liu F, Liu X, Powell CA, et al. Initiative of clinical single-cell biomedicine in clinical and translational medicine. Clin Transl Med. 2023;13(1):e1173.
CrossRef Google scholar
[6]
Wang X, Fan J. Spatiotemporal molecular imaging is a critical part of spatiotemporal molecular medicine. Clin Transl Med. 2021;11(3):e347.
CrossRef Google scholar
[7]
Liu X, Wang DC, Powell CA, Wang X. Challenges of clinical translation from single-cell sequencing to measures in clinical biochemistry of haematology: definition of immune cell identities. Clin Transl Med. 2023;13(9):e1401.
CrossRef Google scholar
[8]
Liu X, Powell CA, Wang X. Forward single-cell sequencing into clinical application: understanding of cancer microenvironment at single-cell solution. Clin Transl Med. 2022;12(4):e782.
CrossRef Google scholar
[9]
Yan F, Li Z, Powell CA, Wang X. Forward single-cell sequencing into clinical application: understanding of ageing and rejuvenation from clinical observation to single-cell solution. Clin Transl Med. 2022;12(5):e827.
CrossRef Google scholar
[10]
Zhang Z, Zhou J, Tan P, et al. Epigenomic diversity of cortical projection neurons in the mouse brain. Nature. 2021;598(7879):167-173.
CrossRef Google scholar
[11]
Lv X, Li S, Li J, et al. Patterned cPCDH expression regulates the fine organization of the neocortex. Nature. 2022;612(7940):503-511.
CrossRef Google scholar
[12]
Yao Z, van Velthoven CTJ, Kunst M, et al. A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain. Nature. 2023;624(7991):317-332.
CrossRef Google scholar
[13]
Duran RC, Wei H, Wu JQ. Single-cell RNA-sequencing of the brain. Clin Transl Med. 2017;6(1):20.
CrossRef Google scholar
[14]
Sun HF, Li LD, Lao IW, et al. Single-cell RNA sequencing reveals cellular and molecular reprograming landscape of gliomas and lung cancer brain metastases. Clin Transl Med. 2022;12(11):e1101.
CrossRef Google scholar
[15]
Xu K, Wang H, Zou YX, et al. Distinct fibroblast subpopulations associated with bone, brain or intrapulmonary metastasis in advanced non-small-cell lung cancer. Clin Transl Med. 2024;14(3):e1605.
CrossRef Google scholar
[16]
Zhang Y, Xing X, Long B, et al. A spatial and cellular distribution of rabies virus infection in the mouse brain revealed by fMOST and single-cell RNA sequencing. Clin Transl Med. 2022;12(1):e700.
CrossRef Google scholar
[17]
Chen D, Ou Z, Zhu J, et al. Screening of cell–virus, cell-–cell, gene–gene crosstalk among animal kingdom at single cell resolution. Clin Transl Med. 2022;12(8):e886.
CrossRef Google scholar
[18]
Li J, Zhang Y, Yang C, Rong R. Discrepant mRNA and protein expression in immune cells. Curr Genomics. 2020;21(8):560-563.
CrossRef Google scholar
[19]
Buccitelli C, Selbach M. mRNAs, proteins and the emerging principles of gene expression control. Nat Rev Genet. 2020;21(10):630-644.
CrossRef Google scholar
[20]
Borm LE, Mossi Albiach A, Mannens CCA, et al. Scalable in situ single-cell profiling by electrophoretic capture of mRNA using EEL FISH. Nat Biotechnol. 2023;41(2):222-231.
CrossRef Google scholar
[21]
Sepp M, Leiss K, Murat F, et al. Cellular development and evolution of the mammalian cerebellum. Nature. 2024;625(7996):788-796.
CrossRef Google scholar
[22]
Sun W, Liu Z, Jiang X, et al. Spatial transcriptomics reveal neuron-astrocyte synergy in long-term memory. Nature. 2024;627(8003):374-381.
CrossRef Google scholar
[23]
Russell AJC, Weir JA, Nadaf NM, et al. Slide-tags enables single-nucleus barcoding for multimodal spatial genomics. Nature. 2024;625(7993):101-109.
CrossRef Google scholar
[24]
Wen X, Luo Z, Zhao W, et al. Single-cell multiplex chromatin and RNA interactions in ageing human brain. Nature. 2024;628:648-656.
CrossRef Google scholar

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