¹⁹F NMR Chemical Shift-switching Probes Enabling Simultaneous Detection of Senescence-associated β-Galactosidase and Monoamine Oxidase A

Abstract

¹⁹F NMR chemical shift-switching probes are highly effective tools for investigating complex biological processes, primarily due to their capacity to produce distinct, crosstalk-free signals from multiple biomarkers. Capitalizing on this capability, we have developed two sets of ¹⁹F NMR probes specifically designed for identifying senescence-associated β-galactosidase (β-gal) and monoamine oxidase A (MAO-A). The probes G-3F and G-F were engineered for β-gal detection, while M-3F, M-2F, and M-F were tailored for MAO-A detection. Among them, probes G-F and M-F demonstrated superior chemical shift changes relative to their series counterparts: G-F’s ¹⁹F signal shifted from δ −77.8 to δ −81.8 after reaction with β-gal, while M-F’s signal shifted from δ −115.7 to δ −109.2 upon encountering MAO-A. Crucially, the resulting signals are well-separated, which facilitates simultaneous detection without mutual interference, as successfully verified in senescent cell models.

Keywords

Molecular probe / ¹⁹F chemical shift / Senescent cell / Biomarker / Simultaneous detection

References

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Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH

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