Alzheimer’s disease (AD) is a progressive neurodegenerative disorder, whose pathogenesis is closely associated with oxidative stress. As a key biomarker of oxidative stress, peroxynitrite (ONOO−) plays a critical role in the onset and progression of AD. Consequently, developing efficient tools for ONOO− detection is crucial for the early diagnosis and pathological investigation of AD. In this study, we designed and synthesized a novel near-infrared (NIR) fluorescent probe, termed CNOP, for the specific recognition and detection of ONOO−. The probe demonstrated excellent optical responses to ONOO−, including high selectivity, outstanding sensitivity (with a detection limit in the nmol/L range), a large Stokes shift, and notably, a significant NIR fluorescence enhancement exceeding 300-fold. Leveraging these superior analytical properties, we successfully applied CNOP for the NIR fluorescence imaging of ONOO− in both live cells and the brains of AD model mice. The imaging results clearly revealed a markedly elevated level of ONOO− in the AD model mice brain compared to that of the wild-type control group, highlighting the probe’s considerable potential for application in AD pathological research.
| [1] |
Kamatham P T, Shukla R, Khatri D K, Vora L K. Ageing Res. Rev., 2024, 101: 102481
|
| [2] |
Eshraghi M, Ahmadi M, Afshar S, Lorzadeh S, Adlimoghaddam A, Rezvani Jalal N, West R, Dastghaib S, Igder S, Torshizi S R N, Mahmoodzadeh A, Mokarram P, Madrakian T, Albensi B C, Los M J, Ghavami S, Pecic S. Pharmacol. Ther., 2022, 237: 108171
|
| [3] |
Ballard C, Aarsland D, Cummings J, O’Brien J, Mills R, Molinuevo J L, Fladby T, Williams G, Doherty P, Corbett A. Nat. Rev. Neurol., 2020, 16: 661
|
| [4] |
Mao L, Han Y, Zhang Q-W, Tian Y. Nat. Commun., 2023, 14: 1419
|
| [5] |
Geng Y J, Wang Z, Zhou J Y, Zhu M G, Liu J, James T D. Chem. Soc. Rev., 2023, 52: 3873
|
| [6] |
Wang X, Chen C, Tian Y, Zhang Q-W. J. Am. Chem. Soc., 2025, 147: 17994
|
| [7] |
Korovesis D, Rubio-Tomas T, Tavernarakis N. Antioxidants, 2023, 12: 131
|
| [8] |
Smith M A, Richey Harris P L, Sayre L M, Beckman J S, Perry G. J. Neurosci., 1997, 17: 2653
|
| [9] |
Ramalingam M, Kim S J. J. Neural. Transm., 2012, 119: 891
|
| [10] |
Torreilles F, Salman-Tabcheh S D, Guérin M-C, Torreilles J. Brain Res. Rev., 1999, 30: 153
|
| [11] |
Ducrocq C, Blanchard B, Pignatelli B, Ohshima H. Cell Mol. Life Sci., 1999, 55: 1068
|
| [12] |
Bai R R, Guo J N, Ye X Y, Xie Y Y, Xie T. Ageing Res. Rev., 2022, 77: 101619
|
| [13] |
Martinez M C, Andriantsitohaina R. Antioxid. Redox. Signal., 2009, 11: 669
|
| [14] |
Ramdial K, Franco M C, Estevez A G. Brain Res. Bull., 2017, 133: 4
|
| [15] |
Perluigi M, Di Domenico F, Butterfield D A. Physiol. Rev., 2024, 104: 103
|
| [16] |
Prolo C, Piacenza L, Radi R. Curr. Opin. Chem. Biol., 2024, 80: 102459
|
| [17] |
McKhann G M, Knopman D S, Chertkow H, Hyman B T, Jack C RJr., Kawas C HJr., Klunk W E, Koroshetz W J, Manly J J, Mayeux R, Mohs R C, Morris J C, Rossor M N, Scheltens P, Carrillo M C, Thies B, Weintraub S, Phelps C H. Alzheimers Dement., 2011, 7: 263
|
| [18] |
Sintini I, Whitwell J L. Curr. Opin. Neurol., 2021, 34: 525
|
| [19] |
Atri A. Med. Clin. North. Am., 2019, 103: 263
|
| [20] |
Wang S, Ren W X, Hou J T, Won M, An J, Chen X, Shu J, Kim J S. Chem. Soc. Rev., 2021, 50: 8887
|
| [21] |
Gu Y Q, Ding J F, Chen J, Xie W N, Xiao L X. Chem. J. Chinese Universities, 2023, 44: 20230367
|
| [22] |
Wang X, Song Y-F, Wan J, Shi Y, Zhang Q-W, Tian Y. Anal. Chem., 2025, 97: 14594
|
| [23] |
Song Y, Tong X, Han Y, Zhang Q-W. Aggregate, 2025, 6: e680
|
| [24] |
Meng Q, Zhang Q-W. Acta Chim. Sinica, 2025, 83: 1252
|
| [25] |
Wang S, Song H, Yin C, Huo F. Coord. Chem. Rev., 2025, 543: 216940
|
| [26] |
Wang X, Ding Q, Groleau R R, Wu L, Mao Y, Che F, Kotova O, Scanlan E M, Lewis S E, Li P, Tang B, James T D, Gunnlaugsson T. Chem. Rev., 2024, 124: 7106
|
| [27] |
Zhu L, Liu D, Wu K, Xie G, Zhao Z, Tang B Z. Chem. Res. Chinese Universities, 2024, 40: 657
|
| [28] |
Chen J Y-H, Shi Q, Peng X, Habimana J D D, Wang J, Sobolewski W, Yeh A H-W. Chem, 2025, 11: 102346
|
| [29] |
Li Y, Wu Z, Huang Z, Yin C, Tian H, Ma X. Natl. Sci. Rev., 2025, 12: nwae383
|
| [30] |
Zhang Q-W, Tian Y. Sci. China Chem., 2023, 66: 923
|
| [31] |
Zheng T, Wang Y, Li X, Li C, Wu P. Chem. Res. Chinese Universities, 2024, 40: 213
|
| [32] |
Wang Z K, Wu L, Wang Y W, Zhang M, Zhao Z Y, Liu C Y, Duan Q X, Jia P, Zhu B C. Anal. Chim. Acta., 2019, 1049: 219
|
| [33] |
Chai X Z, Ma X H, Sun L L, Hu Y Q, Zhang W J, Zhang S Y, Zhou J Q, Zhu L L, Han H H, He X P. Anal. Chem., 2024, 96: 20161
|
| [34] |
Liu H M, Zhu M, Yang H H, Chai L, Han J Y, Ning L J, Zhan Z X. ACS Sens., 2024, 9: 6750
|
| [35] |
Feng B D, Zhong L, Yang Y T, Qu W W, Wang Y F, Wang K, Jiang K, Zhang H. Sens. Actuat. B-Chem., 2025, 423: 136810
|
| [36] |
Zhang D L, Lin B, Han Y F. Sens. Actuat. B-Chem., 2024, 421: 136490
|
| [37] |
Chen C, Pan Y, Li D, Han Y, Zhang Q-W, Tian Y. Anal. Chem., 2022, 94: 6289
|
| [38] |
Gu X, Wang X, Cai W, Han Y, Zhang Q-W. ACS Sens., 2024, 9: 3387
|
| [39] |
Han Y J, Mao L W, Zhang Q W, Tian Y. J. Am. Chem. Soc., 2023, 145: 23832
|
| [40] |
Han Y, Li X, Li D, Chen C, Zhang Q-W, Tian Y. ACS Sens., 2022, 7: 1036
|
| [41] |
Liu J, Wang Z-Q, Mao G-J, Jiang W-L, Tan M, Xu F, Li C-Y. Anal. Chim. Acta, 2021, 1171: 338655
|
| [42] |
Bal-Price A, Matthias A, Brown G C. J. Neurochem., 2002, 80: 73
|
| [43] |
Hashioka S, Han Y H, Fujii S, Kato T, Monji A, Utsumi H, Sawada M, Nakanishi H, Kanba S. Neurochem. Int., 2007, 50: 499
|
| [44] |
Yoo B K, Choi J W, Shin C Y, Jeon S J, Park S J, Cheong J H, Han S Y, Ryu J R, Song M R, Ko K H. Neurochem. Int., 2008, 52: 1188
|
| [45] |
Halasi M, Wang M, Chavan T S, Gaponenko V, Hay N, Gartel A L. Biochem. J., 2013, 454: 201
|
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Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH
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