3D Printed Chitosan Bioink Loaded with Chrysin for Repairing Bone Defects

Abstract

This study aimed to investigate the potential of chrysin (C) to induce osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) in vitro. Chitosan (CS) was dissolved by repeated freeze-thawing of alkali/urea solutions, followed by co-printing of nano-hydroxyapatite (HAP) powders with different concentrations of C solutions (5, 10, and 20 µmol/L) compounded into CS solution. Its rheological properties were modified with acetic acid/gelatin solution to construct C-CS-HAP (C-CS-H) composite scaffolds by extrusion printing technology. Physicochemical characterization showed that the four groups of scaffolds had regularly interconnected porous structures with pore diameters of about 450–600 µm, and the novel bioink exhibited shear-thinning properties, with the viscosity of the material decreasing in the shear rates range of 0.01–1000 s⁻¹ and thus good printability. The osteogenic differentiation ability of BMSCs was confirmed by the CCK-8 test, alkaline phosphatase (ALP) test, Alizarin Red staining (ARS) test, osteogenesis-related genes OCN, ALP, Runx 2, and bone morphogenetic protein-2 (BMP-2) test, which showed a significant promotion of the osteogenic differentiation ability of BMSCs with the increase of the content of C. The above results indicate the potential of C-CS-H composite scaffolds in osteogenesis.

Keywords

Chrysin / Chitosan / Bioscaffold / 3D printing / Hydroxyapatite

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Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH