Differential Antibacterial Effects of Chiral Co₃O₄

Abstract

With the global spread of antimicrobial resistance, it is imperative to satisfy the need to develop novel antibacterial agents. Here, we synthesized chiral Co₃O₄ nanoparticles (NPs) with obvious bactericidal effects. It was found that the circular dichroism (CD) spectra of L-Co₃O₄ NPs and D-Co₃O₄ NPs exhibited perfect mirror image inversion. Specifically, the survival rate of primary uterine fibroblast (PCS)cells reached above 90% by co-culturing with chiral Co₃O₄ NPs. Besides, the chiral Co₃O₄ NPs showed obvious killing ability on Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli O157:H7) bacteria both in vitro and in vivo. Particularly, D-Co₃O₄ NPs showed better antibacterial activity. Mechanistic studies confirmed that the level of reactive oxygen species level generated by D-Co₃O₄ NPs was higher than that of L-Co₃O₄ NPs. Overall, the synthesized D-Co₃O₄ NPs have the potential to become a kind of novel antibacterial nanomaterials.

Keywords

Chiral material / Antibacterial property / Co₃O₄ / Antimicrobial resistance

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