Strategy for Detecting Systemic Treatment Sensitivity of Primary Liver Cancer Based on a Novel Infrared-emissive Organic Nanoparticle

Abstract

In this study, we synthesized an organic material with near-infrared emission capabilities: 4-(2-(4-(9-(4-(diphenylamino) phenyl) naphtho[2,3-c] [1,2,5] thiadiazol-4-yl) phenyl)-1H-phenol-1-ylidene) malononitrile (TPA). Furthermore, TPA-PEG2000 fluorescent nanoparticles were prepared via coating the shells with PEG2000. TPA-PEG2000 exhibited strong near-infrared emission near 700 nm, with a photoluminescence quantum yield of 15.09%, indicating a high emission efficiency. Molecular biology experiments have confirmed its low toxicity and excellent biocompatibility. Increased cholesterol and phospholipid levels in liver cancer cell membranes with low sensitivity or high drug resistance lead to increased rigidity, reduced membrane fluidity, reduced endocytic efficiency, and reduced uptake. Therefore, the uptake of TPA-PEG2000 nanoparticles into cells and the near-infrared fluorescence intensity can be used to evaluate the sensitivity of systemic liver cancer treatment in a simple and efficient manner.

Keywords

Nanoparticle / Near-infrared / Fluorescence / Systemic treatment sensitivity / Cell membrane fluidity

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