Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature

Abstract

Immobilization of enzymes onto porous membranes has attracted considerable attention in recent years. However, enhancing the enzymolysis efficiency of the resulting enzyme reactors by varying the environmental conditions poses a great challenge. In this work, poly(styrene-maleic anhydride-N, N-dimethylacrylamide) was prepared and utilized to construct a thermo-sensitive porous polymer membrane-based enzyme reactor(TS-PPMER) after cellulase was immobilized onto the support by covalent bonding. The catalytic activity of the nano-reactor was evaluated by measuring the yield of the product, glucose, at different temperatures with carboxymethylcellulose as the substrate. Interestingly, the polymer chains coiled and formed numerous nano-pores at a high temperature, which induced the confine effect and greatly boosted the enzymolysis efficiency of TS-PPMER. Furthermore, the proposed TS-PPMER was applied in the hydrolysis of green plant leaves in Epipremnum aureum. This work shows great potential in obtaining biological resources by an environmentally friendly approach using smart polymer-based nano-reactors.

Keywords

Thermo-sensitive porous polymer membrane / Cellulase reactor / Catalytic efficiency / Glucose / Plant leaf

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