Biomass-based Carbon Dots as Peroxidase Mimics for Colorimetric Detection of Glutathione and L-Cysteine

Abstract

Biomass-carbon dots(named as B-CDs) have been successfully prepared via facile one-step hydrothermal reflux treatment at a low temperature from passion fruit peel. The as-prepared B-CDs had a uniform sphere morphology and size of 1–3 nm, which possessed rich nitrogen/oxygen-containing functional groups. B-CDs could imitate peroxidase and accelerate the reaction process of H₂O₂ oxidizing the substrate 3,3’,5,5’-tetramethylbenzidine(TMB), which caused an obvious color change in the solution due to the oxidization of achromic TMB into blue oxidation product(ox-TMB) with an absorption peak at 652 nm. Furthermore, the product could be further reduced to native colorless TMB by the reducing agent glutathione or L-Cysteine. Thus, depending on the peroxidase-like properties of B-CDs, we developed a colorimetric approach to detecting both glutathione and L-Cysteine, which showed superior selective and sensitive detection towards glutathione and L-Cysteine in a linear range of 0–20 µmol/L. The limits of detection were 0.62 and 0.58 µmol/L, respectively.

Keywords

Carbon dot / Biomass / Colorimetry / Glutathione / Cysteine

References

[1] Chaichi M J, Ehsani M, Khajvand T, Golchoubian H, Rezaee E. Spectrochim. Acta A Mol. Biomol. Spectrosc., 2014, 122: 405.

[2] Singh V K, Yadav P K, Chandra S, Bano D, Talat M, Hasan S H. J. Mater. Chem. B, 2018, 6: 5256.

[3] Townsend D M, Tew K D, Tapiero H. Biomed. Pharmacother, 2003, 57: 145.

[4] Mailloux R J, Jin X, Willmore W G. Redox Biol., 2014, 2: 123.

[5] Mahmoud Y I, Sayed S S. Biotech. Histochem., 201, 91: 327.

[6] Spadaro D, Yun B W, Spoel S H, Chu C, Wang Y Q, Loake G J. Physiol. Plant, 2010, 138: 360.

[7] Potesil D, Petrlova J, Adam V, Vacek J, Klejdus B, Zehnalek J, Trnkova L, Havel L, Kizek R. J. Chromatogr. A, 2005, 1084: 134.

[8] Lee P T, Thomson J E, Karina A, Salter C, Johnston C, Davies S G, Compton R G. Analyst, 2015, 140: 236.

[9] Abolghasemi-Fakhri Z, Amjadi M, Manzoori J L. Spectrochim. Acta A: Mol. Biomol. Spectrosc., 2019, 216: 85.

[10] Amjadi M, Abolghasemi-Fakhri Z, Hallaj T. J. Photochem. Photobiol. A, 2015, 309: 8.

[11] Yuan C, Qin X, Xu Y, Li X, Chen Y, Shi R, Wang Y. J. Photochem. Photobiol. A, 2020, 396: 12529.

[12] Hamid M, Khalil R. Food Chem., 2009, 115: 1177.

[13] Gao L, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N, Wang T, Feng J, Yang D, Perrett S, Yan X. Nat. Nanotechnol., 2007, 2: 577.

[14] Hu L, Liao H, Feng L, Wang M, Fu W. Anal. Chem., 2018, 90: 6247.

[15] Vetr F, Moradi-Shoeili Z, Özkar S. J. Organomet. Chem., 2018, 32: e4465.

[16] Mu J, Li J, Zhao X, Yang E C, Zhao X J. Sens. Actuators B: Chem., 2018, 258: 32.

[17] Wang X, Zhou X, Yang K, Li Q, Wan R, Hu G, Ye J, Zhang Y, He J, Gu H, Yang Y, Zhu L. Biomater. Sci., 2021, 9: 2647.

[18] Yang H, Yang R, Zhang P, Qin Y, Chen T, Ye F. Microchim. Acta, 2017, 184: 4629.

[19] Li Z, Liu W, Ni P, Zhang C, Wang B, Duan G, Chen C, Jiang Y, Lu Y. Chem. Eng. J., 2022, 428: 131396.

[20] Wang M, Liu L, Xie X, Zhou X, Lin Z, Su X. Sens. Actuators B: Chem., 2020, 313: 128023.

[21] Wang M, Zhou X, Wang S, Xie X, Wang Y, Su X. Anal. Chem., 2021, 93: 3130.

[22] Wang L, Liu Y, Yang Z, Wang Y, Rao H, Yue G, Wu C, Lu C, Wang X. Dyes Pigm., 2020, 180: 108486.

[23] Yuan C, Qin X, Xu Y, Jing Q, Shi R, Wang Y. Microchem. J., 2020, 159: 105365.

[24] Wei S C, Lin Y W, Chang H T. J. Food Drug Anal., 2020, 28: 559.

[25] Wu J, Wang X, Wang Q, Lou Z, Li S, Zhu Y, Qin L, Wei H. Chem. Soc. Rev., 2019, 48: 1004.

[26] Garg B, Bisht T. Molecules, 201, 21: 1653.

[27] Tong X, Shi S, Tong C, Iftikhar A, Long R, Zhu Y. Trends Analyt. Chem., 2020, 131: 116008.

[28] Wang B L, Zong S Y, Li J Y. Chem. J. Chinese Universities, 2021, 42(1): 299.

[29] Bano D, Kumar V, Singh V K, Chandra S, Singh D K, Yadav P K, Talat M, Hasan S H. ACS Sustain. Chem. Eng., 2018, 7: 1923.

[30] Zhong Q, Chen Y, Su A, Wang Y. Sens. Actuators B: Chem., 2018, 273: 1098.

[31] Luo N, Yang Z, Tang F, Wang D, Feng M, Liao X, Yang X. ACS Appl. Nano Mater., 2019, 2: 3951.

[32] Lu C, Liu Y, Wen Q, Liu Y, Wang Y, Rao H, Shan Z, Zhang W, Wang X. Nanotechnology, 2020, 31: 445703.

[33] Wang Y, Liu X, Wang M, Wang X, Ma W, Li J. Sens. Actuators B: Chem., 2021, 329: 129115.

[34] Chen C Y, Tan Y Z, Hsieh P H, Wang C M, Shibata H, Maejima K, Wang T Y, Hiruta Y, Citterio D, Liao W S. ACS Sens., 2020, 5: 1314.

[35] Zong S, Wang B, Ma W, Yan Y, Li J. Chem. Res. Chinese Universities, 2021, 37(3): 623.

[36] Shu X, Chang Y, Wen H, Yao X, Wang Y. RSC Adv., 2020, 10: 14953.

[37] Tang M, Zhu B, Wang Y, Wu H, Chai F, Qu F, Su Z. Microchim. Acta, 2019, 186: 604.

[38] Zhong Q, Qin X, Yuan C, Shi R, Wang Y. Microchem. J., 2021, 165: 106120.

[39] Yuan C, Qin X, Xu Y, Shi R, Cheng S, Wang Y. Spectrochim. Acta A: Mol. Biomol. Spectrosc., 2021, 254: 119678.

[40] Liang C, Lan Y, Sun Z, Zhou L, Li Y, Liang X, Qin X. Microchim. Acta, 2020, 187: 405.

[41] Zhu D, Zhuo S, Zhu C, Zhang P, Shen W. Anal. Methods, 2019, 11: 2663.

[42] Zhao L, Wu Z, Liu G, Lu H, Gao Y, Liu F, Wang C, Cui J, Lu G. J. Mater. Chem. B, 2019, 7: 7042.

[43] Yadav P K, Singh V K, Chandra S, Bano D, Kumar V, Talat M, Hasan S H. ACS Biomater. Sci. Eng., 2019, 5: 623.

[44] Lu C, Liu X J, Li Y F, Yu F, Tang L H, Hu Y J, Yine Y B. ACS Appl. Mater. Interfaces, 2015, 7: 15395.

[45] Frey A, Meckelein B, Externest D, Schmidt M. J. Immunol. Methods, 2000, 233: 47.